Blockade of IL-6 signaling prevents paclitaxel-induced neuropathy in C57Bl/6 mice
| Autor: | Wolfgang Boehmerle, Hannah Muenzfeld, Matthias Endres, Petra Huehnchen |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Cancer Research metabolism [Interleukin-6] medicine.medical_treatment Pharmacology Neuropathic pain Calcium in biology Cohort Studies chemistry.chemical_compound pathology [Ganglia Spinal] Ganglia Spinal metabolism [Nervous System Diseases] biology lcsh:Cytology pathology [Sensory Receptor Cells] adverse effects [Paclitaxel] Neurodegeneration Calpain Middle Aged Pathophysiology Cytokine Paclitaxel pharmacology [Antibodies Neutralizing] Female Signal Transduction drug effects [Signal Transduction] Sensory Receptor Cells Immunology Models Biological Article Cellular and Molecular Neuroscience Neurotoxicity syndromes ddc:570 medicine Animals Humans lcsh:QH573-671 Chemotherapy Interleukin-6 business.industry pharmacology [Paclitaxel] Cell Biology medicine.disease Antibodies Neutralizing Rats Mice Inbred C57BL chemically induced [Nervous System Diseases] Peripheral neuropathy Animals Newborn chemistry biology.protein Nervous System Diseases drug effects [Sensory Receptor Cells] business |
| Zdroj: | Cell Death & Disease Cell death & disease 11(1), 45 (2020). doi:10.1038/s41419-020-2239-0 Cell Death and Disease, Vol 11, Iss 1, Pp 1-13 (2020) |
| ISSN: | 2041-4889 |
| DOI: | 10.1038/s41419-020-2239-0 |
| Popis: | The microtubule-stabilizing agent paclitaxel frequently leads to chemotherapy-induced peripheral neuropathy (CIN), which further increases the burden of disease and often necessitates treatment limitations. The pathophysiology of CIN appears to involve both “upstream” effects including altered intracellular calcium signaling and activation of calcium dependent proteases such as calpain as well as subsequent “downstream” neuro-inflammatory reactions with cytokine release and macrophage infiltration of dorsal root ganglia. In this study, we aimed to investigate whether these processes are linked by the pro-inflammatory cytokine interleukin-6 (IL-6). We observed that paclitaxel exposure induced IL-6 synthesis in cultured sensory neurons from postnatal Wistar rats, which could be prevented by co-treatment with a calpain inhibitor. This suggests a calcium dependent process. We demonstrate that adult C57BL/6 mice deficient in IL-6 are protected from developing functional and histological changes of paclitaxel-induced neuropathy. Furthermore, pretreatment with an IL-6-neutralizing antibody resulted in the prevention of paclitaxel-induced neuropathy in C57BL/6 mice. Electrophysiological data from our preclinical model was adequately reflected by measurements of patients undergoing paclitaxel therapy for ovarian cancer. In this cohort, measured Il-6 levels correlated with the severity of neuropathy. Our findings demonstrate that IL-6 plays a pivotal role in the pathophysiology of paclitaxel-induced neuropathy per se and that pharmacological or genetic interference with this signaling pathway prevents the development of this potentially debilitating adverse effect. These findings provide a rationale for a clinical trial with IL-6 neutralizing antibodies to prevent dose-limiting neurotoxic adverse effects of paclitaxel chemotherapy. |
| Databáze: | OpenAIRE |
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