Subcellular dynamics of red clover necrotic mosaic virus double-stranded RNAs in infected plant cells
| Autor: | Shota Takata, Kazuyuki Mise, Yoshitaka Takano, Masanori Kaido |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Gene Expression Regulation
Viral Intracellular Space Fluorescent Antibody Technique Dianthovirus Biological Transport Viral replication complex (VRC) Virus Replication Time-Lapse Imaging Viral Proteins Virology Plant Cells Tombusviridae Movement protein Host-Pathogen Interactions RNA Viral Positive-strand RNA virus Endoplasmic reticulum Plant Diseases RNA Double-Stranded |
| Zdroj: | Virology. 568:126-139 |
| ISSN: | 0042-6822 |
| Popis: | New evidences are emerging to support the importance of viral replication complexes (VRCs) in not only viral replication, but also viral cell-to-cell movement. Currently, how VRCs grow in size and colocalize with viral movement proteins (MPs) remains unclear. Herein, we performed live-cell imaging of red clover necrotic mosaic virus (RCNMV) dsRNA by using reporter B2-GFP plants. Tiny granules of dsRNA were formed along the endoplasmic reticulum (ER) at an early stage of infection. Importantly, the colocalization of the dsRNA granules with the virus-encoded p27 replication protein showed that these structures are components of VRCs. These granules moved throughout the cytoplasm, driven by the acto-myosin system, and coalesced with each other to form larger aggregates; the MPs were not associated with these processes. Notably, the MPs colocalized preferentially with large dsRNA aggregates, rather than with tiny dsRNA granules, suggesting that the increase in the size of VRCs promotes their colocalization with MPs. |
| Databáze: | OpenAIRE |
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