Decellularization and oxidation process of bamboo stem enhance biodegradation and osteogenic differentiation
| Autor: | Chakravarthi Mohan, Manitha B. Nair, Unnikrishnan P.S, Aswathy S. H, Amit G Krishnan |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Biocompatibility Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Biomaterials chemistry.chemical_compound Osteogenesis Cellulose Decellularization Tissue Engineering Tissue Scaffolds Sodium periodate Mesenchymal stem cell Biomaterial Cell Differentiation Mesenchymal Stem Cells Adhesion Biodegradation 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Mechanics of Materials Biophysics 0210 nano-technology |
| Zdroj: | Materials scienceengineering. C, Materials for biological applications. 119 |
| ISSN: | 1873-0191 |
| Popis: | Many features that are appropriate for an ideal tissue engineered biomaterial are found in plant tissues. Hierarchically organized Bambusa vulgaris exhibits structural similarities to native bone, but the degradation of cellulose that is the main component of the plant cell wall is a challenge. In this study, Bamboo stem was subjected to decellularization followed by a chemical oxidation process (treated with sodium periodate) to enhance biocompatibility and biodegradation. The crystallinity of oxidised plant scaffolds was reduced, resulting in lower mechanical strength. In contrast, hydrophilicity was enhanced in those scaffolds. In vitro studies demonstrated better mesenchymal stem cell adhesion, viability, and osteogenic differentiation on oxidized scaffolds. Those scaffolds also induced angiogenesis, biocompatibility, and biodegradation when implanted subcutaneously in vivo. Hence, the present study demonstrated the usefulness of "oxidized decellularized plant" as bone scaffold for non-load-bearing applications. |
| Databáze: | OpenAIRE |
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