Application of a chitosan-immobilized Talaromyces thermophilus lipase to a batch biodiesel production from waste frying oils
| Autor: | Zamen Ben Romdhane, Maha Bouzid, Ali Gargouri, Hafedh Belghith, Ines Belhaj-Ben Romdhane |
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| Rok vydání: | 2013 |
| Předmět: |
Bioconversion
Triacylglycerol lipase Bioengineering Environmental pollution complex mixtures Applied Microbiology and Biotechnology Biochemistry Diesel fuel Bioreactors Humans Plant Oils Lipase Molecular Biology Waste Products Biodiesel Chitosan Chromatography biology Esterification Chemistry Methanol General Medicine Transesterification Enzymes Immobilized Talaromyces Biodiesel production Biofuels biology.protein Biotechnology |
| Zdroj: | Applied biochemistry and biotechnology. 171(8) |
| ISSN: | 1559-0291 |
| Popis: | Waste frying oil, which not only harms people’s health but also causes environmental pollution, can be a good alternative to partially substitute petroleum diesel through transesterification reaction. This oil contained 8.8 % of free fatty acids, which cause a problem in a base-catalyzed process. In this study, synthesis of biodiesel was efficiently catalyzed by the covalently immobilized Talaromyces thermophilus lipase and allowed bioconversion yield up to 92 % after 24 h of reaction time. The optimal molar ratio was four to six parts of methanol to one part of oil with a biocatalyst loaded of 25 wt.% of oil. Further, experiments revealed that T. thermophilus lipase, immobilized by a multipoint covalent liaison onto activated chitosan via a short spacer (glutaraldehyde), was sufficiently tolerant to methanol. In fact, using the stepwise addition of methanol, no significant difference was observed from the one-step whole addition at the start of reaction. The batch biodiesel synthesis was performed in a fixed bed reactor with a lipase loaded of 10 g. The bioconversion yield of 98 % was attained after a 5-h reaction time. The bioreactor was operated successfully for almost 150 h without any changes in the initial conversion yield. Most of the chemical and physical properties of the produced biodiesel meet the European and USA standard specifications of biodiesel fuels. |
| Databáze: | OpenAIRE |
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