Duck Plague Virus Promotes DEF Cell Apoptosis by Activating Caspases, Increasing Intracellular ROS Levels and Inducing Cell Cycle S-Phase Arrest
| Autor: | Xiaoyue Chen, Shun Chen, Anchun Cheng, Ying Wu, Xinxin Zhao, Bin Tian, Shaqiu Zhang, Ling Zhang, Dekang Zhu, Mafeng Liu, Chuankuo Zhao, Yunya Liu, Leichang Pan, Qiao Yang, Mujeeb Ur Rehman, Mingshu Wang, Yanling Yu, Renyong Jia |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Embryo Nonmammalian lcsh:QR1-502 Matrix metalloproteinase Polymerase Chain Reaction lcsh:Microbiology Article Flow cytometry Pathogenesis duck plague virus 03 medical and health sciences 0302 clinical medicine Virology medicine Animals Caspase Cells Cultured Membrane Potential Mitochondrial biology medicine.diagnostic_test Chemistry apoptosis ROS Cell Cycle Checkpoints Cell cycle Fibroblasts Cell biology Mitochondria duck embryo fibroblast Mardivirus 030104 developmental biology Infectious Diseases Ducks Viral replication Apoptosis Caspases biology.protein cell cycle Reactive Oxygen Species 030217 neurology & neurosurgery Intracellular |
| Zdroj: | Viruses Viruses, Vol 11, Iss 2, p 196 (2019) Volume 11 Issue 2 |
| ISSN: | 1999-4915 |
| Popis: | Background: Duck plague virus (DPV) can induce apoptosis in duck embryo fibroblasts (DEFs) and in infected ducks, but the molecular mechanism of DPV-induced apoptosis remains unknown. Methods: We first used qRT-PCR and a Caspase-Glo assay to determine whether the caspase protein family plays an important role in DPV-induced apoptosis. Then, we used an intracellular ROS detection kit and the mitochondrial probe JC-1 to respectively detect ROS levels and mitochondrial membrane potential (MMP). Finally, flow cytometry was used to detect apoptosis and cell cycle progression. Results: In this study, the mRNA levels and enzymatic activities of caspase-3, caspase-7, caspase-8, and caspase-9 were significantly increased during DPV-induced apoptosis. The caspase inhibitors Z-DEVD-FMK, Z-LEHD-FMK, and Q-VD-OphA could inhibit DPV-induced apoptosis and promote viral replication. Subsequently, a significant decrease in MMP and an increase in the intracellular ROS levels were observed. Further study showed that pretreating infected cells with NAC (a ROS scavenger) decreased the intracellular ROS levels, increased the MMP, inhibited apoptosis, and promoted viral replication. Finally, we showed that DPV infection can cause cell cycle S-phase arrest. Conclusions: This study shows that DPV causes cell cycle S-phase arrest and leads to apoptosis through caspase activation and increased intracellular ROS levels. These findings may be useful for gaining an understanding of the pathogenesis of DPV and the apoptotic pathways induced by &alpha herpesviruses. |
| Databáze: | OpenAIRE |
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