Mesenchymal Stem Cells from Human Amniotic Membrane and Umbilical Cord Can Diminish Immunological Response in an in vitro Allograft Model
| Autor: | Anna Burdzinska, Leszek Paczek, Aleksandra Zolocinska, Agnieszka Kulesza, Jan Borysowski, Marcin Chlebus, Beata Kaleta, Filip A Dabrowski, Miroslaw Wielgos |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
0301 basic medicine medicine.medical_treatment Adipose tissue Lymphocyte Activation Umbilical cord Umbilical Cord Immunomodulation Andrology 03 medical and health sciences Pregnancy medicine Humans Amnion Lymphocytes Cells Cultured Cell Proliferation Stem cell transplantation for articular cartilage repair business.industry Mesenchymal stem cell Infant Newborn Obstetrics and Gynecology Mesenchymal Stem Cells Amniotic stem cells Allografts Cord lining 030104 developmental biology medicine.anatomical_structure Adipose Tissue Reproductive Medicine Immunology Female business Allotransplantation |
| Zdroj: | Gynecologic and Obstetric Investigation. 82:267-275 |
| ISSN: | 1423-002X 0378-7346 |
| DOI: | 10.1159/000449199 |
| Popis: | Background/Aim: Mesenchymal stem cells (MSCs) are gaining rising interest in gynecology and obstetrics. MSCs immunomodulatory properties are suitable enough to reduce perinatal morbidity caused by inflammation in premature neonates. The aim of this study was to evaluate and compare the ability to inhibit allo-activated lymphocytes proliferation by MSCs derived from different sources: amniotic membrane (AM), umbilical cord (UC) and adipose tissue (AT). Methods: MSCs were isolated from AM (n = 7) and UC (n = 6) and AT (n = 6) of healthy women. Cells were characterized by flow cytometry and differentiation assay. To evaluate the potential of fetal and adult MSCs to diminish immunological response, mixed lymphocytes reaction (MLR) was performed. Results: Amnion and UC-derived cells displayed typical MSCs characteristics. Addition of MSCs to MLR significantly inhibited the proliferation of stimulated lymphocytes. The effect was observed regardless of the MSCs type used (p < 0.01 in all groups). Comparative analysis revealed no significant differences in this action between tested MSCs types. Additionally, no type of MSCs significantly stimulated allogeneic lymphocytes. Conclusion: The results prove the immunosuppressive capacities of fetal-derived MSCs in vitro. In the future, they may be potentially used to treat premature newborn as well as in immunomodulation in post-transplant therapy. |
| Databáze: | OpenAIRE |
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