Efficacy of AdipoDren® in Reducing Interleukin-1-Induced Lymphatic Endothelial Hyperpermeability

Autor: Anna Maidecchi, Lucia Morbidelli, Giulia Antonini, Michela Burico, Luisa Mattoli, Martina Monti, Valerio Ciccone, Francesca Marini
Rok vydání: 2016
Předmět:
0301 basic medicine
Endothelium
Cell Survival
Physiology
Rutin
government.form_of_government
Interleukin-1beta
Inflammation
Pharmacology
Occludin
Tight Junctions
Nitric oxide
Capillary Permeability
03 medical and health sciences
chemistry.chemical_compound
Superoxide Dismutase-1
0302 clinical medicine
medicine
Humans
Lymphedema
Cells
Cultured
NADPH oxidase
Dose-Response Relationship
Drug
biology
Plant Extracts
Endothelial Cells
NADPH Oxidases
Catalase
Endothelial stem cell
Lymphatic Endothelium
030104 developmental biology
Lymphatic system
medicine.anatomical_structure
chemistry
Cyclooxygenase 2
030220 oncology & carcinogenesis
Lymphatic endothelial cells · Permeability · Inflammation ·Plant fractions · Rutin
Immunology
Zonula Occludens-1 Protein
government
biology.protein
Endothelium
Vascular
Plant Preparations
Endothelium
Lymphatic
medicine.symptom
Cardiology and Cardiovascular Medicine
Research Paper
Zdroj: Journal of Vascular Research. 53:255-268
ISSN: 1423-0135
1018-1172
DOI: 10.1159/000452798
Popis: Lymphatic leakage can be seen as a detrimental phenomenon associated with fluid retention and deposition as well as gain of weight. Moreover, lymphatic dysfunction is associated with an inflammatory environment and can be a substrate for other health conditions. A number of treatments can ameliorate lymphatic vasculature: natural substances have been used as treatment options particularly suitable for their consolidated effectiveness and safety profile. Here we report the protective effect of AdipoDren®, an association of a series of plant-derived natural complexes, on lymphatic endothelium permeability promoted by interleukin-1 beta (IL-1β) and the associated molecular mechanisms. AdipoDren® demonstrated a protective effect on dermal lymphatic endothelial cell permeability increased by IL-1β. Reduced permeability was due to the maintenance of tight junctions and cell-cell localisation of occludin and zonula occludens-1 (ZO-1). Moreover, AdipoDren® reduced the expression of the inflammatory key element cyclooxygenase-2 (COX-2), while not altering the levels of endothelial and inducible nitric oxide synthases (eNOS and iNOS). The upregulation of antioxidant enzymatic systems (catalase and superoxide dismutase-1, SOD-1) and the downregulation of pro-oxidant markers (p22 phox subunit of NADPH oxidase) were also evident. In conclusion, AdipoDren® would be useful to ameliorate conditions of altered lymphatic vasculature and to support the physiological functionality of the lymphatic endothelium.
Databáze: OpenAIRE
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