Promiscuous T cell epitope prediction of Candida albicans secretory aspartyl protienase family of proteins
| Autor: | Sansanee C. Chaiyaroj, Songsak Tongchusak, Vladimir Brusic |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Models Molecular Microbiology (medical) Antigens Fungal In silico T cell Molecular Sequence Data Epitopes T-Lymphocyte Virulence Lymphocyte Activation Models Biological Microbiology Epitope Immunomics Candida albicans Consensus Sequence Genetics medicine Aspartic Acid Endopeptidases Humans Amino Acid Sequence Molecular Biology Ecology Evolution Behavior and Systematics Cell Proliferation Sequence Homology Amino Acid biology Secretory Vesicles ELISPOT HLA-DR Antigens Middle Aged biology.organism_classification Virology Corpus albicans Infectious Diseases medicine.anatomical_structure Female HLA-DRB1 Chains |
| Zdroj: | Infection, Genetics and Evolution. 8:467-473 |
| ISSN: | 1567-1348 |
| Popis: | Candida albicans is one of the most important opportunistic dimorphic fungi responsible for hospital acquired fungal infection in humans. Candida infection rarely occurs in healthy individuals but it is frequently associated with patients who suffer from acquired immunodeficiency syndromes. To date, there is no effective vaccine against this fungal infection. Herein we demonstrated the use of immunomics to characterize promiscuous T cell epitope of C. albicans virulence factors by utilizing CandiVF, a C. albicans database previously constructed to be equipped with protein sequence analysis tool, three dimensional structure visualization software, sequence variable analysis program and Hotspot Hunter epitope prediction tool. Secretory aspartyl proteinase (Sap) family was chosen as a model to validate the Hotspot Hunter prediction. Analysis of Saps1-10 protein entries from CandiVF database revealed that a consensus T cell epitope was located at the C-terminal region of Saps1-10. The result of the in silico prediction was subsequently validated by conventional immunological methods. By using overlapping peptides span the predicted consensus T cell epitopes of Saps1-10 as stimulators, it was demonstrated that peptides S6 and S7 could stimulate PBMC proliferation in 9 of 12 blood donors. Interestingly, S2, the predicted T cell epitope of Sap2, was able to induce proliferation of all donors' PBMC. ELISpot assay for the detection of gamma-interferon producing clones confirmed that the peptide S2 actually stimulated T cell proliferation. The results suggest that S2 might be a potential candidate for vaccine development against C. albicans infection or to be utilized as an adjuvant to stimulate the pre-existing CD4+ T cell in other vaccine development. |
| Databáze: | OpenAIRE |
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