In vitro vasorelaxation mechanisms of bioactive compounds extracted from Hibiscus sabdariffa on rat thoracic aorta

Autor: Alassane Wélé, Modou Oumy Kane, Saliou Ngom, Bocar Sarr, Mamadou Sarr, Ramaroson Andriantsitohaina, Lamine Gueye, Doudou Diop, A S Diallo
Přispěvatelé: Laboratoire Physiologie Pharmaceutique, Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD), Environnement, Santé, Sociétés (ESS), Centre National de la Recherche Scientifique (CNRS), Laboratoire de Pharmacognosie et Molécules naturelles bioactives, Laboratoire de Chimie Théapeutique, Laboratoire de Botanique, Institut Fondamental d'Afrique Noire (IFAN), Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD)-Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD), laboratoire de Physiologie Médicale, Biologie Neurovasculaire Intégrée (BNVI), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physiologie Pharmaceutique, BMC, Ed., Unité Mixte Internationale (UMI 3189 - Environnement, Laboratoire de Pharmacognosie et Molécules Naturelles Bioactives, Laboratoire de Chimie Thérapeutique, Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD)-Institut Fondamental d'Afrique Noire (IFAN), Laboratoire de Physiologie Médicale
Jazyk: angličtina
Rok vydání: 2009
Předmět:
[SDV.MHEP.PHY] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]
Endocrinology
Diabetes and Metabolism
[SHS.ANTHRO-BIO]Humanities and Social Sciences/Biological anthropology
Ethyl acetate
Medicine (miscellaneous)
lcsh:TX341-641
030204 cardiovascular system & hematology
High-performance liquid chromatography
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Column chromatography
[SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]
Medicine
Medicinal plants
lcsh:RC620-627
Nutrition and Dietetics
Traditional medicine
business.industry
Research
Hibiscus sabdariffa
Butanol
Senegal
vasorelaxation mechanisms
lcsh:Nutritional diseases. Deficiency diseases
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition
chemistry
Phytochemical
Sephadex
030220 oncology & carcinogenesis
hibiscus sabdariffa
business
lcsh:Nutrition. Foods and food supply
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Zdroj: Nutrition & metabolism
Nutrition & metabolism, 2009, 6 (45), pp.1-12. ⟨10.1186/1743-7075-6-45⟩
Nutrition & metabolism, 2009, 6 (1), pp.45. ⟨10.1186/1743-7075-6-45⟩
Nutrition & Metabolism, Vol 6, Iss 1, p 45 (2009)
Nutrition & Metabolism
DOI: 10.1186/1743-7075-6-45⟩
Popis: Background In this study, we suggested characterizing the vasodilator effects and the phytochemical characteristics of a plant with food usage also used in traditional treatment of arterial high blood pressure in Senegal. Methods Vascular effects of crude extract of dried and powdered calyces of Hibiscus sabdariffa were evaluated on isolated thoracic aorta of male Wistar rats on organ chambers. The crude extract was also enriched by liquid-liquid extraction. The various cyclohexane, dichloromethane, ethyl acetate, butanol extracts obtained as well as the residual marc were subjected to Sephadex LH-20 column chromatography. The different methanolic eluate fractions were then analyzed by Thin Layer (TLC) and High Performance Liquid Chromatography (HPLC) and their vascular effects also evaluated. Results The H. Sabdariffa crude extract induced mainly endothelium-dependent relaxant effects. The endothelium-dependent relaxations result from NOS activation and those who not dependent to endothelium from activation of smooth muscle potassium channels. The phytochemical analysis revealed the presence of phenolic acids in the ethyl acetate extract and anthocyans in the butanolic extract. The biological efficiency of the various studied extracts, in term of vasorelaxant capacity, showed that: Butanol extract > Crude extract > Residual marc > Ethyl acetate extract. These results suggest that the strong activity of the butanolic extract is essentially due to the presence of anthocyans found in its fractions 43-67. Conclusion These results demonstrate the vasodilator potential of hibiscus sabdariffa and contribute to his valuation as therapeutic alternative.
Databáze: OpenAIRE
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