Microtubule architecture in vitro and in cells revealed by cryo-electron tomography
| Autor: | Joseph Atherton, Carolyn A. Moores, Melissa A. Stouffer, Fiona Francis |
|---|---|
| Přispěvatelé: | HAL UPMC, Gestionnaire, Birkbeck College [University of London], Institut du Fer à Moulin (IFM - Inserm U1270 - SU), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Institut National de la Santé et de la Recherche Médicale (INSERM), Cortex development and pathology (U1270), Institut du Fer à Moulin, Sorbonne Université (SU) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Electron Microscope Tomography [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM] Molecular Conformation neurons Context (language use) macromolecular substances Molecular Dynamics Simulation Polymerization microtubules 03 medical and health sciences Structural Biology Microtubule Molecular motor Animals lattice defects Molecular Structure three-dimensional reconstruction [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM] Chemistry Microtubule cytoskeleton Cryoelectron Microscopy Research Papers cryo-electron tomography In vitro 030104 developmental biology Lattice defects Ultrastructure Biophysics Cryo-electron tomography Cattle |
| Zdroj: | Acta crystallographica Section D : Structural biology [1993-...] Acta crystallographica Section D : Structural biology [1993-..], 2018, 74, pp.572-584. ⟨10.1107/S2059798318001948⟩ Acta Crystallographica. Section D, Structural Biology Acta crystallographica. Section D, Structural biology Acta crystallographica. Section D, Structural biology, International Union of Crystallography, 2018, 74, pp.572-584. ⟨10.1107/S2059798318001948⟩ |
| ISSN: | 2059-7983 |
| DOI: | 10.1107/S2059798318001948⟩ |
| Popis: | Electron microscopy is a key methodology for studying microtubule structure and organization. Here, the results of cryo-electron tomography experiments on in vitro-polymerized microtubules and comparisons with microtubule ultrastructure in cells are described. The microtubule cytoskeleton is involved in many vital cellular processes. Microtubules act as tracks for molecular motors, and their polymerization and depolymerization can be harnessed to generate force. The structures of microtubules provide key information about the mechanisms by which their cellular roles are accomplished and the physiological context in which these roles are performed. Cryo-electron microscopy allows the visualization of in vitro-polymerized microtubules and has provided important insights into their overall morphology and the influence of a range of factors on their structure and dynamics. Cryo-electron tomography can be used to determine the unique three-dimensional structure of individual microtubules and their ends. Here, a previous cryo-electron tomography study of in vitro-polymerized GMPCPP-stabilized microtubules is revisited, the findings are compared with new tomograms of dynamic in vitro and cellular microtubules, and the information that can be extracted from such data is highlighted. The analysis shows the surprising structural heterogeneity of in vitro-polymerized microtubules. Lattice defects can be observed both in vitro and in cells. The shared ultrastructural properties in these different populations emphasize the relevance of three-dimensional structures of in vitro microtubules for understanding microtubule cellular functions. |
| Databáze: | OpenAIRE |
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