High level expression of the envelope glycoproteins of the human immunodeficiency virus type I in presence of rev gene using helper-independent adenovirus type 7 recombinants
| Autor: | Pranab K. Chanda, Surendra K. Dheer, Lynda Greenberg, Paul P. Hung, Katherine Molnar-Kimber, Alan R. Davis, Bheem M. Brat, Bruce B. Mason, Michael D. Lubeck, Robert J. Natuk, Satoshi Mizutani |
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| Rok vydání: | 1990 |
| Předmět: |
Gene Expression Regulation
Viral Transcriptional Activation Genes Viral viruses RNA Splicing Genetic Vectors DNA Recombinant Biology Recombinant virus medicine.disease_cause Gp41 law.invention Adenoviridae Viral envelope Viral Envelope Proteins law Virology Gene expression medicine Humans RNA Messenger Gene Glycoproteins Gene Products env rev Gene Products Human Immunodeficiency Virus Molecular biology Genes rev Kinetics Gene Products rev Gene Products tat Recombinant DNA HIV-1 RNA Viral tat Gene Products Human Immunodeficiency Virus Expression cassette HeLa Cells |
| Zdroj: | Virology. 175(2) |
| ISSN: | 0042-6822 |
| Popis: | The effect of rev (art/trs) gene on the level of HIV-1 envelope (env) expression using recombinant adenovirus was investigated. Recombinant adenoviruses expressing either the envelope or the rev gene of the human immunodeficiency virus type 1 (HIV-1) were constructed by inserting the gene into an expression cassette. The expression cassette contained the adenovirus type 7 major late promoter, followed by leader 1 of the adenovirus tripartite leader and a portion of intron between leaders 1 and 2, leaders 2 and 3, and a hexon polyadenylation signal. The cassette was then inserted at the terminal region between the E4 and ITR regions of the adenovirus 7 genome with a concomitant E3 region deletion (80–87 m.u.). A549 cells infected with the recombinant virus containing the env gene produced the envelope glycoproteins gpl60, gpl20, and gp41. HIV-1 envelope gene expression was greatly enhanced (20-to 50-fold) in the cells that were simultaneously infected with the recombinant adenovirus containing the rev gene as measured by ELISA and Western blotting. Interestingly, this effect was observed despite the lack of the 5′ down splice site for rev and seems to be post-transcriptional. Another recombinant adenovirus which contains both the rev and the env genes was constructed by inserting the rev gene in the deleted E3 region and the env gene in the terminal cassette. This double recombinant virus expressed high levels of env antigen in A549 cells similar to those attained upon co-infection with two separate recombinant viruses containing the rev or env gene. Furthermore, the rev gene nucleotide sequence could be altered without altering the amino acid sequence and its sequences truncated by 17 amino acids from the C-terminus had no effect of rev function. |
| Databáze: | OpenAIRE |
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