Cytotoxicity of temporary cements on bovine dental pulp-derived cells (bDPCs) using realtime cell analysis
| Autor: | Sema S. Hakki, Serife Buket Bozkurt, Abdulkadir Sengun, Meral Arslan Malkoç, Necla Demİr |
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| Přispěvatelé: | Kırıkkale Üniversitesi, Selçuk Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | The Journal of Advanced Prosthodontics |
| ISSN: | 2005-7814 2005-7806 |
| Popis: | WOS: 000350184300004 PubMed: 25722833 PURPOSE. To evaluate the cytotoxicity of temporary luting cements on bovine dental pulp-derived cells (bDPCs). MATERIALS AND METHODS. Four different temporary cements were tested: Rely X Temp E (3M ESPE), Ultratemp (Ultradent), GC Fuji Temp (GC), and Rely X Temp NE (3M ESPE). The materials were prepared as discs and incubated in Dulbecco's modified eagle's culture medium (DMEM) for 72 hours according to ISO 10993-5. A real-time cell analyzer was used to determine cell vitality. After seeding 200 mu L of the cell suspensions into the wells of a 96-well plate, the bDPCs were cured with bioactive components released by the test materials and observed every 15 minutes for 98 hours. One-way ANOVA and Tukey-Kramer tests were used to analyze the results of the proliferation experiments. RESULTS. All tested temporary cements showed significant decreases in the bDPCs index. Rely X Temp E, GC Fuji Temp, and Rely X Temp NE were severely toxic at both time points (24 and 72 hours) (P.05); however, the cell viability was significantly reduced at 72 hours (P |
| Databáze: | OpenAIRE |
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