Cytogenetic analysis of Hypomasticus copelandii and H. steindachneri: relevance of cytotaxonomic markers in the Anostomidae family (Characiformes)
| Autor: | Filipe Schitini Salgado, Marina Souza da Cunha, Jorge Abdala Dergam, Silvana Melo |
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| Přispěvatelé: | Universidade Federal de Viçosa (UFV), Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Anostomidae Anastomid medicine.medical_specialty food.ingredient repetitive sequences Plant Science Characiformes QH426-470 010603 evolutionary biology 01 natural sciences cytogenetics 03 medical and health sciences food coastal basins Molecular Cytogenetics medicine Genetics Megaleporinus Animalia Chordata fluorescence in situ hybridization biology medicine.diagnostic_test Actinopterygii Cytogenetics endemic species Chromosome Karyotype biology.organism_classification freshwater fishes 030104 developmental biology Evolutionary biology Microsatellite Animal Science and Zoology Hypomasticus Brazil Biotechnology Fluorescence in situ hybridization Research Article |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Comparative Cytogenetics 15(1): 65-76 Comparative Cytogenetics, Vol 15, Iss 1, Pp 65-76 (2021) Comparative Cytogenetics |
| Popis: | Made available in DSpace on 2021-06-25T12:40:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-03-09 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) Recent phylogenetic hypotheses within Anostomidae, based on morphological and molecular data, resulted in the description of new genera (Megaleporinus Ramirez, Birindelli et Galetti, 2017) and the synonymization of others, such as the reallocation of Leporinus copelandii Steindachner, 1875 and Leporinus steindachneri Eigenmann, 1907 to Hypomasticus Borodin, 1929. Despite high levels of conservatism of the chromosomal macrostructure in this family, species groups have been corroborated using banding patterns and the presence of different sex chromosome systems. Due to the absence of cytogenetic studies in H. copelandii (Steindachner, 1875) and H. steindachneri (Eigenmann, 1907), the goal of this study was to characterize their karyotypes and investigate the presence/absence of sex chromosome systems using different repetitive DNA probes. Cytogenetic techniques included: Giemsa staining, Ag-NOR banding and FISH using 18S and 5S rDNA probes, as well as microsatellite probes (CA)(15) and (GA)(15). Both species had 2n = 54, absence of heteromorphic sex chromosomes, one chromosome pair bearing Ag-NOR, 18S and 5S rDNA regions. The (CA)(15) and (GA)(15) probes marked mainly the subtelomeric regions of all chromosomes and were useful as species-specific chromosomal markers. Our results underline that chromosomal macrostructure is congruent with higher systematic arrangements in Anostomidae, while microsatellite probes are informative about autapomorphic differences between species. Univ Fed Vicosa, Dept Biol Anim, Ave PH Rolfs S-N, BR-36570900 Vicosa, MG, Brazil Univ Fed Vicosa, Dept Biol Geral, Ave PH Rolfs S-N, BR-36570900 Vicosa, MG, Brazil Univ Estadual Paulista, Inst Biociencias, Dept Morfol, R Prof Dr Antonio Celso Wagner Zanin S-N, BR-18618689 Botucatu, SP, Brazil Univ Estadual Paulista, Inst Biociencias, Dept Morfol, R Prof Dr Antonio Celso Wagner Zanin S-N, BR-18618689 Botucatu, SP, Brazil |
| Databáze: | OpenAIRE |
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