Nanospray analysis of the venom of the tarantulaTheraphosa leblondi: a powerful method for direct venom mass fingerprinting and toxin sequencing
| Autor: | Marie-Louise Célérier, Christian Legros, Maud Henry, Catherine Guette |
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| Rok vydání: | 2004 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Electrospray Potassium Channels Protein mass spectrometry Molecular Sequence Data Spider Venoms Venom In Vitro Techniques Mass spectrometry medicine.disease_cause Peptide Mapping Mass Spectrometry Analytical Chemistry Potassium Channel Blockers medicine Animals Amino Acid Sequence Spectroscopy Tarantula Chromatography biology Edman degradation Chemistry Toxin Organic Chemistry Spiders biology.organism_classification Theraphosa Shal Potassium Channels Potassium Channels Voltage-Gated Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization |
| Zdroj: | Rapid Communications in Mass Spectrometry. 18:1024-1032 |
| ISSN: | 1097-0231 0951-4198 |
| DOI: | 10.1002/rcm.1442 |
| Popis: | Mass spectrometric methods, including matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS), on-line liquid chromatography/electrospray ionisation mass spectrometry (LC/ESI-MS), and nanospray ionisation/hybrid quadrupole time-of-flight mass spectrometry (nanoESI-QqTOFMS), were applied to characterize by mass fingerprinting the venom of the French Guyanese tarantula Theraphosa leblondi. Of these techniques direct nanoESI-QqTOFMS, which allowed the detection of 65 protonated molecules with high mass accuracy, appeared to give the best results. Three major peptides, TlTx1, TlTx2 and TlTx3, were sequenced using a combination of nanoESI-MS/MS and enzyme digestion/MS and MS/MS experiments. Each sequence was confirmed by automated Edman sequencing. In patch-clamp experiments these peptides were found to have a specific inhibitory effect on the voltage-dependent potassium channel, Kv4.2. |
| Databáze: | OpenAIRE |
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