Superior Osteo-Inductive and Osteo-Conductive Properties of Trabecular Titanium vs. PEEK Scaffolds on Human Mesenchymal Stem Cells: A Proof of Concept for the Use of Fusion Cages
| Autor: | Fulvio Tartara, Alberto Romano, Natale Francaviglia, Elena De Vecchi, Laura de Girolamo, Enrico Ragni, Alessandro Bidossi, Carlotta Perucca Orfei, Gianluca Maestretti |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Scaffold
Polymers cage 02 engineering and technology cervical spine Calcium deposition Polyethylene Glycols lcsh:Chemistry 0302 clinical medicine Peek lcsh:QH301-705.5 Spectroscopy Fusion Tissue Scaffolds osteo-conduction lumbar spine Cell Differentiation Prostheses and Implants General Medicine Ketones Middle Aged Computer Science Applications Alkaline phosphatase Female Titanium Adult arthrodesis 0206 medical engineering chemistry.chemical_element osteo-induction Article Catalysis osteogenesis Inorganic Chemistry Benzophenones 03 medical and health sciences PEEK Humans titanium Physical and Theoretical Chemistry Molecular Biology mesenchymal stem cells Organic Chemistry Mesenchymal stem cell 020601 biomedical engineering In vitro Gene Expression Regulation chemistry lcsh:Biology (General) lcsh:QD1-999 030217 neurology & neurosurgery Biomedical engineering |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 2379, p 2379 (2021) International Journal of Molecular Sciences Volume 22 Issue 5 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Fusion cages composed of titanium and its alloys are emerging as valuable alternative to standard polyetheretherketone (PEEK) ones routinely used in cervical and lumbar spine surgery. Aim of this study was to evaluate osteo-inductive and osteo-conductive ability of an innovative trabecular titanium (T-Ti) scaffold on human mesenchymal stem cells (hMSCs), in both absence and presence of biochemical osteogenic stimuli. Same abilities were assessed on PEEK and standard 2D plastic surface, the latter meant as gold-standard for in vitro differentiation studies. hMSCs adhered and colonized both T-Ti and PEEK scaffolds. In absence of osteogenic factors, T-Ti triggered osteogenic induction of MSCs, as demonstrated by alkaline phosphatase activity and calcium deposition increments, while PEEK and standard 2D did not. Addition of osteogenic stimuli reinforced osteogenic differentiation of hMSCs cultured on T-Ti in a significantly higher manner with respect to standard 2D plastic culture surfaces, whereas PEEK almost completely abolished the process. T-Ti driven differentiation towards osteoblasts was confirmed by gene and marker expression analyses, even in absence of osteogenic stimuli. These results clearly indicate superior in vitro osteo-inductive and osteo-conductive capacity of T-Ti compared to PEEK, and make ground for further studies supporting the use of T-Ti cages to improve bone fusion. |
| Databáze: | OpenAIRE |
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