Characterization of resistance genes in multidrug-resistant Salmonella enterica serotype Typhimurium isolated from diseased cattle in France (2002 to 2007)
| Autor: | Axel Cloeckaert, Cécile Ponsin, Danièle Meunier, Hayette Targant, Benoît Doublet, Christiane Brunet, Jean-Yves Madec |
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| Přispěvatelé: | Inconnu, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA) |
| Rok vydání: | 2010 |
| Předmět: |
Serotype
Salmonella typhimurium Salmonella Genomic Islands [SDV]Life Sciences [q-bio] Cattle Diseases Drug resistance Microbial Sensitivity Tests Biology medicine.disease_cause Integron Applied Microbiology and Biotechnology Microbiology Polymerase Chain Reaction Integrons Foodborne Diseases 03 medical and health sciences Antibiotic resistance Species Specificity Drug Resistance Multiple Bacterial medicine Animals Cluster Analysis Serotyping Bacteriophage Typing ComputingMilieux_MISCELLANEOUS 030304 developmental biology Phage typing 0303 health sciences Salmonella Infections Animal 030306 microbiology Nucleotide Mapping Genetic Variation biology.organism_classification Virology 3. Good health Electrophoresis Gel Pulsed-Field Multiple drug resistance Salmonella enterica Genes Bacterial biology.protein Animal Science and Zoology Cattle France Genes MDR Food Science |
| Zdroj: | Foodborne Pathogens and Disease Foodborne Pathogens and Disease, Mary Ann Liebert, 2010, 7 (4), pp.419-425. ⟨10.1089/fpd.2009.0414⟩ |
| ISSN: | 1556-7125 1535-3141 |
| DOI: | 10.1089/fpd.2009.0414⟩ |
| Popis: | We report here the results of the survey of antimicrobial resistance in 148 serotype Typhimurium strains isolated from cattle in France from 2002 to 2007 and displaying more than two antimicrobial resistances. Salmonella enterica serotype Typhimurium of definitive phage type 104 strains that are commonly resistant to ampicillin-amoxicillin, chloramphenicol-florfenicol, streptomycin-spectinomycin, sulfonamides, and tetracycline (ACSSuT phenotype) harbored resistance genes clustered on a complex class 1 integron In104 of the Salmonella genomic island 1 (SGI1). In our isolates, the most common antimicrobial resistance pattern was ACSSuT (77.7%) or ACSSuT combined to additional resistances. SGI1 was detected in 143 strains and constituted thus the main structure involved in resistance to antimicrobials in these strains. In spite of the high recombination potential of In104, SGI1 variability was quite limited among these strains since only two SGI1 variants, SGI1-B and SGI1-C, were identified. One hundred and thirty-eight out of the 143 SGI1-positive isolates belonged to the DT104 complex. Pulsed-field gel electrophoresis profile A was the most prevalent in 135 SGI1-positive isolates, confirming the diffusion of the DT104 clone. However, changes in phages susceptibility have occurred in three serotype Typhimurium strains of phage type DT12, as they displayed the same pulsed-field gel electrophoresis profile as the SGI1-positive serotype Typhimurium DT104. No variant harboring an additional resistance gene was identified, but the risk of recombination between SGI1 and any other mobile structure carrying other antimicrobial resistance genes is still an issue in serotype Typhimurium. |
| Databáze: | OpenAIRE |
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