Identification of an ATP-binding cassette transport system required for translocation of lipopolysaccharide O-antigen side-chains across the cytoplasmic membrane of Klebsiella pneumoniae serotype O1
| Autor: | Dorothea Bronner, Bradley R. Clarke, Chris Whitfield |
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| Rok vydání: | 1994 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Biological Transport Active ATP-binding cassette transporter Biology Microbiology Adenosine Triphosphate Plasmid ATP hydrolysis Gene cluster Amino Acid Sequence Cloning Molecular Serotyping Codon Microscopy Immunoelectron Molecular Biology Integral membrane protein Peptide sequence Base Sequence Sequence Homology Amino Acid Cell Membrane Polysaccharides Bacterial Chromosome Mapping O Antigens Klebsiella pneumoniae Carbohydrate Sequence Biochemistry Genes Bacterial Multigene Family Membrane topology ATP-Binding Cassette Transporters Bacterial outer membrane |
| Zdroj: | Molecular Microbiology. 14:505-519 |
| ISSN: | 1365-2958 0950-382X |
| DOI: | 10.1111/j.1365-2958.1994.tb02185.x |
| Popis: | The rfbKpO1 gene cluster of Klebsiella pneumoniae O1 directs synthesis of the D-galactan I component of the lipopolysaccharide O-antigen. The first two genes in the rfbKpO1 cluster encode RfbAKpO1 and RfbBKpO1, with predicted sizes of 29.5 or 30.0 kDa and 27.4 kDa, respectively. RfbBKpO1 contains a consensus ATP-binding domain and shares homology with several proteins which function as ATP-binding components of cell surface polysaccharide transporters. RfbAKpO1 is predicted to be an integral membrane protein with five putative membrane-spanning domains and its transmembrane topology was confirmed by TnphoA mutagenesis. The hydropathy plot of RfbAKpO1 resembles KpsM, the transcytoplasmic membrane component of the capsular polysaccharide transporter from Escherichia coli K-1 and K-5. These relationships suggest that RfbAKpO1 and RfbBKpO1 belong to a family of two-component ABC (ATP-binding cassette) transporters. E. coli K-12 containing a plasmid carrying an rfbKpO1 gene cluster deleted in rfbAKpO1 and rfbBKpO1 expresses rough lipopolysaccharide molecules on its surface and accumulates cytoplasmic O-antigen. When RfbAKpO1 and RfbBKpO1 are supplied in trans by a compatible plasmid, O-polysaccharide transport is restored and smooth D-galactan I-substituted lipopolysaccharide is produced. RfbAKpO1 and RfbBKpO1 are, therefore, proposed to constitute a system required for transport of D-galactan I across the cytoplasmic membrane, where RfbAKpO1 represents the membrane-spanning translocator and RfbBKpO1 couples the energy of ATP hydrolysis ot the transport process. |
| Databáze: | OpenAIRE |
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