Serine utilization in mouse liver: influence of caloric restriction and aging
| Autor: | Kevork Hagopian, Richard Weindruch, Jon J. Ramsey |
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| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty Aging L-Serine Dehydratase Glycerate dehydrogenase activity Biophysics Serine dehydratase Biology Biochemistry Transaminase Serine Mice Structural Biology Internal medicine Serine:pyruvate transaminase Genetics medicine Animals Glycerate dehydrogenase Molecular Biology Transaminases Caloric Restriction Gluconeogenesis Cell Biology Pyruvate dehydrogenase complex Mice Inbred C57BL Phosphotransferases (Alcohol Group Acceptor) Endocrinology Liver Glycerate kinase |
| Zdroj: | FEBS letters. 579(9) |
| ISSN: | 0014-5793 |
| Popis: | The influence of caloric restriction (CR) on the activities of hepatic serine metabolizing enzymes in young (3 months) and old (30 months) mice was studied. Serine dehydratase (SDH) activity increased markedly with age in both diet groups and in old mice was higher in the CR group. No effects of CR were observed in the young. Serine:pyruvate transaminase (SPT) and glycerate kinase activities were unaffected by age and diet. However, glycerate dehydrogenase activity was decreased in old CR mice but not in young CR. The results of this study show that long-term CR influenced serine utilization only in the pathway catalyzed by SDH. This suggests that in mouse liver this pathway is critical for serine utilization in gluconeogenesis, while the SPT pathway plays a minor role. The increase in SDH activity with long-term CR is consistent with sustained increase in gluconeogenesis. |
| Databáze: | OpenAIRE |
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