Neutrophils cause oxidative DNA damage in alveolar epithelial cells
Autor: | Paul J. A. Borm, Peter Nehls, P.A.E.L. Schilderman, Roel P. F. Schins, Joachim Bruch, Frank Seiler, Ad M. Knaapen |
---|---|
Přispěvatelé: | Gezondheidsrisico Analyse en Toxicologie, RS: NUTRIM School of Nutrition and Translational Research in Metabolism |
Jazyk: | angličtina |
Rok vydání: | 1999 |
Předmět: |
Male
Neutrophils DNA damage Inflammation medicine.disease_cause Biochemistry Pathogenesis chemistry.chemical_compound Physiology (medical) medicine Animals Humans Hydrogen peroxide Cells Cultured chemistry.chemical_classification Reactive oxygen species Lung Epithelial Cells Hydrogen Peroxide Molecular biology Rats Pulmonary Alveoli medicine.anatomical_structure chemistry Immunology Immunohistochemistry medicine.symptom Carcinogenesis Oxidation-Reduction DNA Damage |
Zdroj: | Free Radical Biology and Medicine, 27(1-2), 234-240. Elsevier Science |
ISSN: | 0891-5849 |
Popis: | Department of Health Risk Analysis and Toxicology, Maastricht University, The Netherlands.Inflammation has been recognized as a contributing factor in the pathogenesis of some cancers. In the lung, inflammation is characterized by an influx of polymorphonuclear leukocytes (PMN) that release a variety of reactive oxygen species (ROS). The aim of the present study was to investigate the direct effect of PMN on oxidative DNA damage in lung target cells. Therefore, rat alveolar epithelial cells (RLE) were coincubated with PMN or hydrogen peroxide. Known to be correlated with the incidence of cancer, 7-hydro-8-oxo-2'deoxyguanosine (8-oxodG) was used as an effect marker for oxidative damage. Viability of the RLE, when coincubated with PMN, decreased to 43%, dependent on the ratio between PMN and RLE. After washing off PMN, 8-oxodG levels were significantly increased in RLE, but the highest levels were observed in the washed off PMN fraction. In addition, to avoid washing off procedures, immunohistochemical analysis was used to measure the 8-oxodG levels specifically in the RLE and similar results were obtained. In addition, inhibitor experiments showed that antioxidants ameliorated oxidative DNA damage. Our data provide evidence that ROS released by PMN as well as H2O2, cause oxidative DNA damage in epithelial cells. |
Databáze: | OpenAIRE |
Externí odkaz: |