Increased expression of Toll-like receptors 2, 3, 4 and 7 mRNA in the kidney and intestine of a septic mouse model
| Autor: | Diamantis I. Tsilimigras, Anastasios Machairas, Alkistis Kapelouzou, Michalis Katsimpoulas, Evangelos P. Misiakos, Manolis Mavroidis, Theodore Liakakos, Dimitrios Schizas, Theano Demesticha, Stylianos Vagios, Sylvia Krivan, Demetrios Moris, Chrysostomos V Aravanis, Kitty Pavlakis |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine lcsh:Medicine Peritonitis Kidney Article Sepsis Mice 03 medical and health sciences 0302 clinical medicine Immune system medicine Animals RNA Messenger lcsh:Science Receptor Cecum Ligation Multidisciplinary business.industry lcsh:R Toll-Like Receptors Acute kidney injury Acute Kidney Injury medicine.disease Acquired immune system 3. Good health Mice Inbred C57BL Disease Models Animal 030104 developmental biology medicine.anatomical_structure Immunology Immunohistochemistry lcsh:Q business 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-9 (2019) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-40537-2 |
| Popis: | Toll-like receptors (TLRs) are the key regulators of innate and adaptive immunity and are highly expressed during sepsis. Thus, studying the expression of TLRs in an animal septic model might indicate their possible association with acute kidney injury in sepsis. Seventy-two male C57BL/6J mice were used for this study. Randomly, these animals were divided into 6 groups (N = 12/group): 3 control and 3 septic groups depending on the euthanasia time (24 h, 48 h, 72 h). Septic groups underwent cecal ligation and puncture (CLP) to induce peritonitis, while control groups had a sham operation. Hematological tests were performed in serum for immune biomarkers; immunohistochemistry, morphometry and qRT-PCR analysis were used on both kidney and intestine tissues to evaluate the expression of TLR 2, 3, 4 and 7 in a septic process. At the end of each experimental period, we found that TLRs 2, 3, 4 and 7 were expressed in both tissues but there were differences between those at various time points. Also, we found that mRNA levels were significantly higher in qRT-PCR evaluation in septic groups than control groups in both kidney and intestinal tissues (p |
| Databáze: | OpenAIRE |
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