Andrology: An indiscriminate use of pentoxifylline does not improve in-vitro fertilization in poor fertilizers
| Autor: | Paul Devroey, Andre Van Steirteghem, Herman Tournaye, G. Verheyen, M. Camus, Ronny Janssens |
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| Přispěvatelé: | Department of Embryology and Genetics, Vrije Universiteit Brussel, Centre for Reproductive Medicine - Gynaecology |
| Rok vydání: | 1994 |
| Předmět: |
Adult
Male endocrine system medicine.medical_treatment Acrosome reaction Fertilization in Vitro Biology Andrology Human fertilization medicine Humans Prospective Studies Pentoxifylline Infertility Male Sperm motility In vitro fertilisation Hyperactivation Rehabilitation Povidone Obstetrics and Gynecology Silicon Dioxide Spermatozoa Sperm Embryo transfer Culture Media Reproductive Medicine Sperm Motility Female Percoll |
| Zdroj: | Vrije Universiteit Brussel Europe PubMed Central |
| ISSN: | 1460-2350 0268-1161 |
| DOI: | 10.1093/oxfordjournals.humrep.a138696 |
| Popis: | In order to enhance fertilization in vitro, pentoxifylline (PTX) was used in couples showing low fertilization rates in previous in-vitro fertilization (IVF) attempts for the treatment of male-factor infertility. Sibling oocytes were inseminated at random with spermatozoa prepared with or without PTX. After selection by Percoll gradient, sperm samples were divided into two equal aliquots. One aliquot was incubated in Earle's medium containing approximately 3.6 mM PTX (treatment group), the other aliquot was incubated with PTX-free Earle's medium (control group). After 30 min, both suspensions were washed twice. Sperm parameters after preparation did not differ between treatment and control samples, and nor did the mean fertilization rates, which were 49.3 and 42.6% respectively. Cleavage characteristics and morphological quality of the embryos were not significantly different between the treatment and control groups. The results of this study demonstrate that indiscriminate use of PTX in an IVF programme increases neither fertilization rate nor embryo transfer rate in poor fertilizers. More prospective research is needed to evaluate the role of PTX in IVF in couples selected according to the effect of the compound on sperm function, e.g. hyperactivation and acrosome reaction. |
| Databáze: | OpenAIRE |
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