Electrochemically-assisted deposition of biomimetic hydroxyapatite–collagen coatings on titanium plate
| Autor: | Giorgio Tosi, Elisabetta Foresti, Piera Sabatino, Massimo Marcaccio, Silvia Manara, Francesco Paolucci, Simona Sabbatini, Barbara Palazzo, Norberto Roveri, George Altankov |
|---|---|
| Přispěvatelé: | S. Manara, F. Paolucci, B. Palazzo, M. Marcaccio, E. Foresti, G.Tosi, S. Sabbatini, P. Sabatino, G. Altankov, N. Roveri |
| Rok vydání: | 2008 |
| Předmět: |
Chemistry
FIBRONECTIN BINDING HYDROXYAPATITE–COLLAGEN COATING Composite number Analytical chemistry Nucleation engineering.material Apatite LASER SCANNING CONFOCAL MICROSCOPY Inorganic Chemistry BIOMIMETIC CRYSTAL GROWTH Adsorption Chemical engineering Coating Fibronectin binding Nanocrystal ELECTROCHEMICALLY-ASSISTED DEPOSITION visual_art Materials Chemistry engineering visual_art.visual_art_medium Physical and Theoretical Chemistry Infrared microscopy |
| Zdroj: | Inorganica Chimica Acta. 361:1634-1645 |
| ISSN: | 0020-1693 |
| Popis: | A biomimetic bone-like composite, made of self-assembled collagen fibrils and carbonate hydroxyapatite nanocrystals, has been performed by an electrochemically-assisted deposition on titanium plate. The electrolytic processes have been carried out using a single type I collagen molecules suspension in a diluted Ca(NO3)2 and NH4H2PO4 solution at room temperature and applying a constant current for different periods of time. Using the same electrochemical conditions, carbonate hydroxyapatite nanocrystals or reconstituted collagen fibrils coatings were obtained. The reconstituted collagen fibrils, hydroxyapatite nanocrystals and collagen fibrils/apatite nanocrystals coatings have been characterized chemically, structurally and morphologically, as well as for their ability to bind fibronectin (FN). Fourier Transform Infrared microscopy has been used to map the topographic distribution of the coating components at different times of electrochemical deposition, allowing to single out the individual deposition steps. Moreover, roughness of Ti plate has been found to affect appreciably the nucleation region of the inorganic nanocrystals. Laser scanning confocal microscopy has been used to characterize the FN adsorption pattern on a synthetic biomimetic apatitic phase, which exhibits a higher affinity when it is inter-grown with the collagen fibrils. The results offer auspicious applications in the preparation of medical devices such as biomimetic bone-like compositecoated metallic implants. _ 2007 Elsevier B.V. All rights reserved. Keywords: Hydroxyapatite–collagen coating; Electrochemically-assisted deposition; Micro-imaging FTIR spectroscopy; Laser scanning confocal microscopy; Biomimetic crystal growth; Fibronectin binding |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect