Molecular characterization of Cryptosporidium spp. in poultry from Brazil
| Autor: | Maria Júlia Rodrigues da Cunha, Mónica Santín, Márcia Cristina Cury |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Veterinary medicine animal structures 030231 tropical medicine Cryptosporidiosis Cryptosporidium Pheasant Zoonotic disease Poultry 03 medical and health sciences Feces 0302 clinical medicine biology.animal Zoonoses parasitic diseases Animals Humans Poultry Diseases Genetic diversity General Veterinary biology Transmission (medicine) 030108 mycology & parasitology biology.organism_classification Subtyping Quail embryonic structures Chickens Brazil |
| Zdroj: | Research in veterinary science. 118 |
| ISSN: | 1532-2661 |
| Popis: | Cryptosporidiosis is an important zoonotic disease caused by Cryptosporidium. Infections in birds are mainly caused by C. meleagridis, C. baileyi, and C. galli. C. meleagridis is the third most common cause of cryptosporidiosis in humans and the only Cryptosporidium species known to infect both birds and mammals. One hundred and fifty-five fecal specimens from different poultry species (chicken, turkey, ostrich, helmeted guinea fowl, quail, pheasant, and emu) were collected at local markets in the state of Minas Gerais, Brazil. Twenty-three (14.8%) birds (20 chickens, 2 quails, and 1 turkey) were found Cryptosporidium-positive. This constitutes the first report of Cryptosporidium in turkeys from Brazil. Nucleotide sequence analysis identified C. meleagridis in chickens (15), a turkey (1), and a quail (1), C. baileyi in chickens (4) and a quail (1), and a mixed infection C. meleagridis/C. baileyi in a chicken (1). This is the first report of C. meleagridis in turkeys and quails from Brazil. Using the gp60 gene, three subtype families were identified, IIIa, IIIb and IIIg. Within subtype family IIIg, four subtypes were identified in chickens, two novel (IIIgA25G3R1 and IIIgA21G3R1) and two previously reported (IIIgA22G3R1 and IIIgA24G2R1). Within subtype family IIIb two subtypes were identified, IIIbA24G1R1 in a chicken and IIIbA23G1R1 in a quail. A novel subtype in the family IIIa was identified (IIIaA22G3R1) in a turkey. The finding of C. meleagridis subtypes previously identified in humans (IIIgA22G3R1, IIIbA24G1R1 and IIIbA23G1R1) indicates that they can be potentially zoonotic. Further subtyping studies that clarify genetic diversity of C. meleagridis are required to better understand host specificity, source of infection, and transmission dynamics of C. meleagridis. |
| Databáze: | OpenAIRE |
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