Loss of major nutrient sensing and signaling pathways suppresses starvation lethality in electron transport chain mutants
| Autor: | Rebecca Y Wang, Jason V. Rogers, Maria Ingaramo, R. Scott McIsaac, Alisha G Lewis, Ilya Soifer, Patrick A. Gibney, David G. Hendrickson, Robert L. Caldwell, David Botstein |
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| Rok vydání: | 2021 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Mutant Saccharomyces cerevisiae Cell Biology Nutrient sensing Oxidative phosphorylation Hydrogen-Ion Concentration Biology Phenotype Electron transport chain Cell biology Electron Transport Metabolic pathway Cytosol Glucose Gene Expression Regulation Fungal Genome Mitochondrial Mutation Signal transduction Inner mitochondrial membrane Molecular Biology Signal Transduction Transcription Factors |
| Zdroj: | Molecular Biology of the Cell. 32 |
| ISSN: | 1939-4586 1059-1524 |
| DOI: | 10.1091/mbc.e21-06-0314 |
| Popis: | The electron transport chain (ETC) is a well-studied and highly conserved metabolic pathway that produces ATP through generation of a proton gradient across the inner mitochondrial membrane coupled to oxidative phosphorylation. ETC mutations are associated with a wide array of human disease conditions and to aging-related phenotypes in a number of different organisms. In this study, we sought to better understand the role of the ETC in aging using a yeast model. A panel of ETC mutant strains that fail to survive starvation was used to isolate suppressor mutants that survive. These suppressors tend to fall into major nutrient sensing and signaling pathways, suggesting that the ETC is involved in proper starvation signaling to these pathways in yeast. These suppressors also partially restore ETC-associated gene expression and pH homeostasis defects, though it remains unclear whether these phenotypes directly cause the suppression or are simply effects. This work further highlights the complex cellular network connections between metabolic pathways and signaling events in the cell and their potential roles in aging and age-related diseases. |
| Databáze: | OpenAIRE |
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