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PDF file - 263K, Effects of EMD 1214063 on c-Met phosphorylation and downstream signaling molecules (see Figure 2C). EBC-1 cells were cultured in vitro in control medium (medium + vehicle) (lane 1), or in the presence of increasing concentrations of EMD 1214063 (A-D) or EMD 1204831(E-H) (0.01nM to 30�M, as depicted in lanes 2-15). Upon cell lysis, the levels of total c-Met, AKT, p42/44 MAPK (A, E), Gab1 (C, G), as well as phospho-c-Met, phospho-Akt, phospho-p42/44 MAPK (B, F), and phospho-Gab1 (D, H) were assessed by Western blot analyses. The levels of cofilin were used to ascertain equal loading (A, B, and F). Marked reductions in the levels of c-Met phosphorylation were observed for EMD 1214063 and EMD 1204831 at concentrations ≥0.1 �M (lanes 10-15). Data are representative of two independent experiments; cumulative results are shown in Fig. 2C. |
