Acaricidal Activity of Petroleum Ether Extract of Leaves of Tetrastigma leucostaphylum (Dennst.) Alston against Rhipicephalus (Boophilus) annulatus

Autor: Suresh Narayanan Nair, N. D. Chithra, K. P. Sreelekha, Sanis Juliet, P. E. Deepa, Reghu Ravindran, K.G. Ajith Kumar, T. P. Ajeesh Krishna, T. P. Adarsh Krishna, Srikanta Ghosh, U. Darsana
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: The Scientific World Journal
The Scientific World Journal, Vol 2014 (2014)
ISSN: 1537-744X
2356-6140
Popis: The acaricidal activity of the petroleum ether extract of leaves ofTetrastigma leucostaphylum(Dennst.) Alston (family: Vitaceae) againstRhipicephalus (Boophilus) annulatuswas assessed using adult immersion test (AIT). The per cent of adult mortality, inhibition of fecundity, and blocking of hatching of eggs were studied at different concentrations. The extract at 10% concentration showed 88.96% inhibition of fecundity, 58.32% of adult tick mortality, and 50% inhibition of hatching. Peak mortality rate was observed after day 5 of treatment. Mortality of engorged female ticks, inhibition of fecundity, and hatching of eggs were concentration dependent. The LC50value of the extract againstR. (B.) annulatuswas 10.46%. The HPTLC profiling of the petroleum ether extract revealed the presence of at least seven polyvalent components. In the petroleum ether extract, nicotine was identified as one of the components up to a concentration of 5.4%. However, nicotine did not reveal any acaricidal activity up to 20000 ppm (2%). Coconut oil, used as diluent for dissolving the extract, did not reveal any acaricidal effects. The results are indicative of the involvement of synergistic or additive action of the bioactive components in the tick mortality and inhibition of the oviposition.
Databáze: OpenAIRE