The TOM Complex of Amoebozoans: the Cases of the Amoeba Acanthamoeba castellanii and the Slime Mold Dictyostelium discoideum
| Autor: | Malgorzata Slocinska, Hanna Kmita, Olgierd Stobienia, Dorota Buczek, Andonis Karachitos, Wojciech Makalowski, Monika Antoniewicz, Małgorzata Wojtkowska |
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| Rok vydání: | 2015 |
| Předmět: |
Acanthamoeba castellanii
Sequence Homology Amino Acid biology Translocase of the outer membrane translocase of the outer membrane Lobosa phylogenesis TIM/TOM complex Dictyostelium discoideum biology.organism_classification Microbiology Conosa Amoebozoa Cell biology mitochondria Protein Subunits TOM complex Mitochondrial Precursor Protein Import Complex Proteins Slime mold Dictyostelium Carrier Proteins |
| Zdroj: | Protist. 166:349-362 |
| ISSN: | 1434-4610 |
| DOI: | 10.1016/j.protis.2015.05.005 |
| Popis: | Protein import into mitochondria requires a wide variety of proteins, forming complexes in both mitochondrial membranes. The TOM complex (translocase of the outer membrane) is responsible for decoding of targeting signals, translocation of imported proteins across or into the outer membrane, and their subsequent sorting. Thus the TOM complex is regarded as the main gate into mitochondria for imported proteins. Available data indicate that mitochondria of representative organisms from across the major phylogenetic lineages of eukaryotes differ in subunit organization of the TOM complex. The subunit organization of the TOM complex in the Amoebozoa is still elusive, so we decided to investigate its organization in the soil amoeba Acanthamoeba castellanii and the slime mold Dictyostelium discoideum. They represent two major subclades of the Amoebozoa: the Lobosa and Conosa, respectively. Our results confirm the presence of Tom70, Tom40 and Tom7 in the A. castellanii and D. discoideum TOM complex, while the presence of Tom22 and Tom20 is less supported. Interestingly, the Tom proteins display the highest similarity to Opisthokonta cognate proteins, with the exception of Tom40. Thus representatives of two major subclades of the Amoebozoa appear to be similar in organization of the TOM complex, despite differences in their lifestyle. |
| Databáze: | OpenAIRE |
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