Identification and quantification of cereulide in cow's milk using liquid chromatography-tandem mass spectrometry
| Autor: | Akihiko Hirai, Yoko Matsushima, Hiroshi Koike, Kotaro Sekimura, Chieko Nagano, Tetsuya Shindo, Maki Kanda, Hiroshi Hayashi, Yukiko Nakagawa, Takeo Sasamoto, Tsuneo Hashimoto, Yumi Ohba, Junichi Kamiie |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Health Toxicology and Mutagenesis 030106 microbiology Food Contamination Toxicology Mass spectrometry 03 medical and health sciences chemistry.chemical_compound Milk products Liquid chromatography–mass spectrometry Tandem Mass Spectrometry Depsipeptides Animals Chromatography High Pressure Liquid Chromatography Public Health Environmental and Occupational Health food and beverages General Chemistry General Medicine Repeatability Cereulide Raw milk Milk chemistry Calibration Cattle Food Science |
| Zdroj: | Food additivescontaminants. Part A, Chemistry, analysis, control, exposurerisk assessment. 35(12) |
| ISSN: | 1944-0057 |
| Popis: | In this study, the presence of cereulide in cow's milk was identified and quantified using our validated method with liquid chromatography-tandem mass spectrometry. Cereulide was concentrated using protein acid-precipitation and extracted from the precipitate by using acetonitrile twice. The combination of protein acid-precipitation and extraction sufficiently eliminated the matrix compounds from the milk and a further clean-up step utilising solid-phase extraction could be omitted. For robustly measuring the samples and keeping the MS devices clean, the extraction solution was diluted 10-fold using methanol. Owing to the minimisation of the interferences caused by fragmentation patterns, multiple reaction monitoring information-dependent acquisition-enhanced product ion spectra enabled the characterisation and identification of cereulide. Besides the matrix effect (-4%), an external solvent calibration curve was adapted for accurate quantification. The method was validated using fortified recovery tests, at two concentrations (10 and 50 µg kg |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |