Comparative genome-wide polymorphic microsatellite markers in Antarctic penguins through next generation sequencing
| Autor: | Daly Noll, Paulina Valenzuela-Guerra, Gisele P. M. Dantas, Daniel González-Acuña, David E. Loyola, Isidora Mura-Jornet, Cristell Navarro, Juliana A. Vianna |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Evolutionary Genetics
0301 basic medicine lcsh:QH426-470 Population Biology tetranucleotide Genome DNA sequencing polymorphism 03 medical and health sciences Abundance (ecology) Genetics Polymorphic Microsatellite Marker education Molecular Biology genome education.field_of_study penguin microsatellite markers biology.organism_classification Pygoscelis lcsh:Genetics 030104 developmental biology Evolutionary biology Genetic marker Microsatellite |
| Zdroj: | Genetics and Molecular Biology, Volume: 40, Issue: 3, Pages: 676-687, Published: SEP 2017 Genetics and Molecular Biology v.40 n.3 2017 Genetics and Molecular Biology Sociedade Brasileira de Genética (SBG) instacron:SBG Genetics and Molecular Biology, Vol 40, Iss 3, Pp 676-687 |
| Popis: | Microsatellites are valuable molecular markers for evolutionary and ecological studies. Next generation sequencing is responsible for the increasing number of microsatellites for non-model species. Penguins of the Pygoscelis genus are comprised of three species: Adélie (P. adeliae), Chinstrap (P. antarcticus) and Gentoo penguin (P. papua), all distributed around Antarctica and the sub-Antarctic. The species have been affected differently by climate change, and the use of microsatellite markers will be crucial to monitor population dynamics. We characterized a large set of genome-wide microsatellites and evaluated polymorphisms in all three species. SOLiD reads were generated from the libraries of each species, identifying a large amount of microsatellite loci: 33,677, 35,265 and 42,057 for P. adeliae, P. antarcticus and P. papua, respectively. A large number of dinucleotide (66,139), trinucleotide (29,490) and tetranucleotide (11,849) microsatellites are described. Microsatellite abundance, diversity and orthology were characterized in penguin genomes. We evaluated polymorphisms in 170 tetranucleotide loci, obtaining 34 polymorphic loci in at least one species and 15 polymorphic loci in all three species, which allow to perform comparative studies. Polymorphic markers presented here enable a number of ecological, population, individual identification, parentage and evolutionary studies of Pygoscelis, with potential use in other penguin species. |
| Databáze: | OpenAIRE |
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