Katanin-Dependent Microtubule Ordering in Association with ABA Is Important for Root Hydrotropism
| Autor: | Rui Miao, Wei Siao, Na Zhang, Zuliang Lei, Deshu Lin, Rishikesh P. Bhalerao, Congming Lu, Weifeng Xu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Arabidopsis Proteins
Organic Chemistry Arabidopsis Biochemistry and Molecular Biology Water General Medicine Microtubules Plant Roots Tropism Catalysis Computer Science Applications Inorganic Chemistry cortical microtubule arrays oryzalin KATANIN abscisic acid root hydrotropism Physical and Theoretical Chemistry Katanin Molecular Biology Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 7; Pages: 3846 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms23073846 |
| Popis: | Root hydrotropism refers to root directional growth toward soil moisture. Cortical microtubule arrays are essential for determining the growth axis of the elongating cells in plants. However, the role of microtubule reorganization in root hydrotropism remains elusive. Here, we demonstrate that the well-ordered microtubule arrays and the microtubule-severing protein KATANIN (KTN) play important roles in regulating root hydrotropism in Arabidopsis. We found that the root hydrotropic bending of the ktn1 mutant was severely attenuated but not root gravitropism. After hydrostimulation, cortical microtubule arrays in cells of the elongation zone of wild-type (WT) Col-0 roots were reoriented from transverse into an oblique array along the axis of cell elongation, whereas the microtubule arrays in the ktn1 mutant remained in disorder. Moreover, we revealed that abscisic acid (ABA) signaling enhanced the root hydrotropism of WT and partially rescued the oryzalin (a microtubule destabilizer) alterative root hydrotropism of WT but not ktn1 mutants. These results suggest that katanin-dependent microtubule ordering is required for root hydrotropism, which might work downstream of ABA signaling pathways for plant roots to search for water. |
| Databáze: | OpenAIRE |
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