QUANTITATION OF RYANODINE RECEPTOR OF RABBIT SKELETAL-MUSCLE, HEART AND BRAIN
| Autor: | Alfredo Margreth, Gianantonio Tobaldin, Pompeo Volpe, Ernesto Damiani |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
Biophysics Biology Biochemistry Myofibrils Chaps Internal medicine medicine Animals Receptors Cholinergic Terminal cisternae Molecular Biology Differential centrifugation Lagomorpha Ryanodine Ryanodine receptor Muscles Myocardium Endoplasmic reticulum Cardiac muscle Brain Skeletal muscle Ryanodine Receptor Calcium Release Channel Cell Biology biology.organism_classification SARCOPLASMIC-RETICULUM Kinetics Sarcoplasmic Reticulum medicine.anatomical_structure Endocrinology Rabbits |
| Popis: | The total number of high-affinity ryanodine receptor (RyR) binding sites present in skeletal and cardiac muscle and in brain tissue of the rabbit was determined by [ 3 H]ryanodine binding to subfractions obtained by differential centrifugation of homogenates prepared in a low-ionic strength medium, containing 0.5% Chaps. In all three tissues at least 80% of [ 3 H]ryanodine binding was recovered in the total membrane ( TM ) fraction obtained by centrifuging between 650 g for 10 min and 120,000 × g for 90 min. Skeletal muscle displayed higher contents of high-affinity RyR sites (about 49 pmol/g wet wt) than heart and brain (about 12 pmol and 3.5 pmol/g wet wt, respectively). The affinity for ryanodine, as well as the affinity for Ca 2+ , in the absence or presence of Ca 2+ -releasing drugs (caffeine and doxorubicin) of TM from skeletal muscle, were found to be identical to those of purified terminal cisternae. As low as 1 g of tissue was sufficient to perform several experiments. |
| Databáze: | OpenAIRE |
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