Study on the optical and biological properties in vitro ofIR808‐PEG‐FA
| Autor: | Shengtao Fang, Wang Sujun, Yanli Fan, Wenjing Feng, Qianqian Tang, Chunmeng Shi, Jie Du, Huiyuan Ya |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Cell Survival medicine.medical_treatment 0206 medical engineering Biomedical Engineering Photodynamic therapy 02 engineering and technology Polyethylene Glycols Biomaterials chemistry.chemical_compound Folic Acid In vivo Neoplasms PEG ratio medicine Humans Fluorescent Dyes Optical Imaging technology industry and agriculture Metals and Alloys 021001 nanoscience & nanotechnology 020601 biomedical engineering In vitro Light intensity Photochemotherapy chemistry A549 Cells Folate receptor MCF-7 Cells Ceramics and Composites Biophysics 0210 nano-technology Ethylene glycol Conjugate |
| Zdroj: | Journal of Biomedical Materials Research Part A. 108:1816-1823 |
| ISSN: | 1552-4965 1549-3296 |
| DOI: | 10.1002/jbm.a.36946 |
| Popis: | IR808, an IR780 derivative, is capable of fluorescently imaging and photodynamic therapy in vitro and in vivo. However, its application is greatly hampered by hydrophobicity, toxicity and nonspecific delivery to the targeting tissue and that causes accumulation in the liver and kidney. In order to overcome these limitations, we prepared IR808-PEG-FA from IR808, amino-terminated poly(ethylene glycol) (NH2 -PEG-NH2 , denoted as PEG) and folate (FA). PEG, an accepted hydrophilic medicinal agent, was introduced to improve hydrophobicity, and FA was used to increase targeting ability of the conjugate. The obtained product provides a good water solubility and stronger light intensity in near infrared (NIR)-imaging, and CCK-8 test demonstrated which had no appreciable toxicity. In addition, the cell uptake results indicated that IR808-PEG-FA was specifically targeted to positive tumors cells with folate receptor (FR) compared with IR808, and thus it may be used as a novel diagnostic agent or imaging-guided agent for cancer treatment. So this article provides a way to improve hydrophobicity, optical stability and targeting ability in the field of nano-probe for fluorochromes. |
| Databáze: | OpenAIRE |
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