Effect of Anthocyanins Contained in a Blackberry Extract on the Circulatory Failure and Multiple Organ Dysfunction Caused by Endotoxin in the Rat
| Autor: | Sautebin, L, Rossi, A, Serraino, I, Dugo, Paola, DI PAOLA, R, Mondello, Luigi, Genovese, Tiziana, Britti, D, Peli, A, Dugo, Giovanni, Caputi, Achille, Cuzzocrea, Salvatore, DI PAOLA, Rosanna |
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| Přispěvatelé: | L. SAUTEBIN, A. ROSSI, I. SERRAINO, P. DUGO, R. DI PAOLA, L. MONDELLO, T. GENOVESE, D. BRITTI, A. PELI, G. DUGO, A.P. CAPUTI, S. CUZZOCREA, Sautebin, Lidia, Rossi, Antonietta, Serraino, I., Dugo, P., DI PAOLA, R., Mondello, L., Genovese, T., Britti, D., Peli, A., Dugo, G., Caputi, A. P., Cuzzocrea, S. |
| Rok vydání: | 2004 |
| Předmět: |
Lipopolysaccharides
Male Lipopolysaccharide Bilirubin Multiple Organ Failure Nitric Oxide Synthase Type II Vaccinium myrtillus Pharmaceutical Science Pharmacology Biology Analytical Chemistry Nitric oxide Transaminase Anthocyanins Rats Sprague-Dawley Lipid peroxidation chemistry.chemical_compound Ileum Drug Discovery Animals ENDOTOXIC SHOCK Enzyme Inhibitors Lung Plant Extracts Nitrotyrosine Organic Chemistry BLACKBERRY food and beverages PEROXYNITRITE Malondialdehyde Rats Endotoxins Isoenzymes Disease Models Animal Complementary and alternative medicine chemistry Biochemistry Cyclooxygenase 2 Prostaglandin-Endoperoxide Synthases Fruit Anthocyanin INOS COX2 Molecular Medicine Nitric Oxide Synthase Phytotherapy |
| Zdroj: | Planta Medica. 70:745-752 |
| ISSN: | 1439-0221 0032-0943 |
| Popis: | Anthocyanins are a group of naturally occurring phenolic compounds related to the colouring of plants, flowers and fruits. These pigments are important as quality indicators, chemotaxonomic markers and for their antioxidant activities. Here we have investigated the therapeutic efficacy of anthocyanins contained in a blackberry extract on (i) circulatory failure, (ii) multiple organ dysfunction and (iii) activity of the inducible isoforms of nitric oxide (NO) synthase (iNOS) and cyclooxygenase (COX-2) in anaesthetised rats with endotoxic shock. In a model of endotoxic shock induced by lipopolysaccharide (LPS, E. coli, 10 mg/kg, i. v.) in the rat, pretreatment with anthocyanins present in the blackberry extract (5 mg/kg, i. v. 30 min before LPS) prevented the hypotension induced by LPS. Endotoxaemia also caused rises in the serum levels of (i) glutamyl oxaloacetic transaminase (GOT), glutamyl pyruvic transaminase (GPT), alkaline phosphates and bilirubin (hepatic dysfunction) (ii) creatinine (renal dysfunction), (iii) amylase and lipase (pancreatic injury), (iiii) NOx and 6-keto-PGF1α. Anthocyanins attenuated the hepatic and pancreatic injury, the renal dysfunction and decreased NOx and 6-keto-PGF1α levels. Endotoxaemia for 6 h resulted in a substantial increase in iNOS and COX activity in rat lung, which was attenuated in rats pretreated with anthocyanins. Moreover, anthocyanins (0.02 - 0.32 mg/mL) inhibited in vitro iNOS and COX activity from lung of LPS-treated rats. Polymorphonuclear (PMN) infiltration (myeloperoxidase activity), lipid peroxidation (malondialdehyde levels) as well as tissue injury (histological examination) induced by LPS in rat lung and ileum was reduced by anthocyanins (5 mg/kg, i. v. 30 min before LPS). Furthermore, endotoxaemia induced the formation of nitrotyrosine and poly(ADP-ribose) synthetase (PARS) activation as determined by immunohistochemical analysis of lung and ileum tissues. The degree of staining was lowered by anthocyanin treatment. These results indicate that the anthocyanins contained in the blackberry extract exert multiple protective effects in endotoxic shock. |
| Databáze: | OpenAIRE |
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