CENP-B Cooperates with Set1 in Bidirectional Transcriptional Silencing and Genome Organization of Retrotransposons
| Autor: | Lauren F Meyer, Anastasia Berg, Peter Johansen, Irina V. Mikheyeva, Shiv I. S. Grewal, Hugh P. Cam, David R. Lorenz |
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| Rok vydání: | 2012 |
| Předmět: |
Retroelements
Transcription Genetic Amino Acid Motifs Retrotransposon Biology Histone Deacetylases Gene Expression Regulation Fungal Schizosaccharomyces Sirtuins Gene Silencing Molecular Biology Transcription factor Genomic organization Genetics Gene Expression Profiling Terminal Repeat Sequences Articles Histone-Lysine N-Methyltransferase Cell Biology biology.organism_classification Long terminal repeat Chromatin DNA-Binding Proteins Histone Schizosaccharomyces pombe Histone Methyltransferases biology.protein Schizosaccharomyces pombe Proteins Genome Fungal Centromere Protein B Transcription Factors |
| Zdroj: | Molecular and Cellular Biology. 32:4215-4225 |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.00395-12 |
| Popis: | Regulation of transposable elements (TEs) is critical to the integrity of the host genome. The fission yeast Schizosaccharomyces pombe homologs of mammalian CENP-B perform a host genome surveillance role by controlling Tf2 long terminal repeat (LTR) retrotransposons. However, the mechanisms by which CENP-Bs effect their functions are ill defined. Here, we show that the multifaceted roles of Abp1, the prominent member of fission yeast CENP-Bs, are mediated in part via recognition of a 10-bp AT-rich motif present in most LTRs and require the DNA-binding, transposase, and dimerization domains of Abp1 to maintain transcriptional repression and genome organization. Expression profiling analyses indicated that Abp1 recruits class I/II histone deacetylases (HDACs) to repress Tf2 retrotransposons and genes activated in response to stresses. We demonstrate that class I/II HDACs and sirtuins mediate the clustering of dispersed Tf2 retrotransposons into Tf bodies. Intriguingly, we uncovered an unexpected cooperation between Abp1 and the histone H3K4 methyltransferase Set1 in regulating sense and antisense transcriptional silencing of Tf2 retrotransposons and Tf body integrity. Moreover, Set1-mediated regulation of Tf2 expression and nuclear organization appears to be largely independent of H3K4 methylation. Our study illuminates a molecular pathway involving a transposase-containing transcription factor that cooperates with chromatin modifiers to regulate TE activities. |
| Databáze: | OpenAIRE |
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