Feasibility of using multiphoton excited tissue autofluorescence for in vivo human histopathology
| Autor: | Rebecca M. Williams, Johanna M. Dela Cruz, Warren R. Zipfel, Jesse D. McMullen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Pathology ocis:(180.4315) Nonlinear microscopy Biology Gene mutation 01 natural sciences law.invention 010309 optics 03 medical and health sciences In vivo law 0103 physical sciences Microscopy medicine Irradiation 030304 developmental biology 0303 health sciences Laser Atomic and Molecular Physics and Optics Excited state Histopathology ocis:(180.0180) Microscopy Preclinical imaging Biotechnology Biomedical engineering |
| Zdroj: | Biomedical Optics Express |
| ISSN: | 2156-7085 |
| Popis: | Rapid and direct imaging of microscopic tissue morphology and pathology can be achieved by multiphoton imaging of intrinsic tissue fluorophores and second harmonic signals. Engineering parameters for developing this technology for clinical applications include excitation levels and collection efficiencies required to obtain diagnostic quality images from different tissue types and whether these levels are mutagenic. Here we provide data on typical average powers required for high signal-to-noise in vivo tissue imaging and assess the risk potential of these irradiance levels using a mammalian cell gene mutation assay. Exposure times of ~16 milliseconds per cell to 760 nm, ~200 fs raster-scanned laser irradiation delivered through a 0.75 NA objective produced negligible mutagenicity at powers up to about 50 mW. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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