Identification of putative ecdysteroid and juvenile hormone pathway genes in the shrimp Neocaridina denticulata
| Autor: | Ricky Wai Tak Leung, Ka Hou Chu, Yung Wa Sin, Nathan J. Kenny, Ka Wo Chan, Jerome H.L. Hui, Stephen S. Tobe, Sam P. S. Cheong, Ting-Fung Chan, Katie W.S. Chan, Zhe Qu, William G. Bendena |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
animal structures Juvenile-hormone esterase Molecular Sequence Data Retinoid X receptor Biology Real-Time Polymerase Chain Reaction chemistry.chemical_compound Endocrinology Decapoda Internal medicine medicine Animals RNA Messenger Regulator gene Ecdysteroid Hormone binding protein Reverse Transcriptase Polymerase Chain Reaction fungi Ecdysteroids Juvenile Hormones Biochemistry chemistry Juvenile hormone Animal Science and Zoology Ecdysone receptor Signal Transduction Hormone |
| Zdroj: | General and Comparative Endocrinology. 214:167-176 |
| ISSN: | 0016-6480 |
| DOI: | 10.1016/j.ygcen.2014.07.018 |
| Popis: | Although the sesquiterpenoid juvenile hormone (JH) and the steroidal ecdysteroids are of vital importance to the development and reproduction of insects, our understanding of the evolution of these crucial hormonal regulators in other arthropods is limited. To better understand arthropod hormone evolution and regulation, here we describe the hormonal pathway genes (e.g. those involved in hormone biosynthesis, degradation, regulation and signal transduction) of a new decapod model, the shrimp Neocaridina denticulata. The majority of known insect sesquiterpenoid and ecdysteroid pathway genes and their regulators are contained in the N. denticulata genome. In the sesquiterpenoid pathway, these include biosynthetic pathway components: juvenile hormone acid methyltransferase (JHAMT); hormone binding protein: juvenile hormone binding protein (JHBP); and degradation pathway components: juvenile hormone esterase (JHE), juvenile hormone esterase binding protein (JHEBP) and juvenile hormone epoxide hydrolase (JHEH), with the JHBP, JHEBP and JHEH genes being discovered in a crustacean for the first time here. Ecdysteroid biosynthetic pathway genes identified include spook, phantom, disembodied, shadow and CYP18. Potential hormonal regulators and signal transducers such as allatostatins (ASTs), Methoprene-tolerant (Met), Retinoid X receptor (RXR), Ecdysone receptor (EcR), calponin-like protein Chd64, FK509-binding protein (FKBP39), Broad-complex (Br-c), and crustacean hyperglycemic hormone/molt-inhibiting hormone/gonad-inhibiting hormone (CHH/MIH/GIH) genes are all present in the shrimp N. denticulata. To our knowledge, this is the first report of these hormonal pathways and their regulatory genes together in a single decapod, providing a vital resource for further research into development, reproduction, endocrinology and evolution of crustaceans, and arthropods in general. |
| Databáze: | OpenAIRE |
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