Comparison of the activation peptide regions of protease-activated receptors (PAR) in members of the Family Felidae
| Autor: | Sara Madison Barnes, Mary K. Boudreaux |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Felidae medicine.medical_treatment Receptors Proteinase-Activated Biology 03 medical and health sciences medicine Animals Humans Coding region Nucleotide Amino Acid Sequence Receptor Peptide sequence chemistry.chemical_classification Genetics Protease Base Sequence General Veterinary Nucleic acid sequence Amino acid genomic DNA 030104 developmental biology chemistry Biochemistry Cats Peptides |
| Zdroj: | Veterinary Clinical Pathology. 45:400-405 |
| ISSN: | 0275-6382 |
| Popis: | Background There is limited information regarding the nucleotides encoding or the predicted amino acid composition of protease-activated receptors (PAR) in cats. Objectives The purpose of the study was to determine the nucleotide sequence and predicted amino acid composition of the activation peptide regions of protease-activated receptors PAR1, PAR3, and PAR4 in Felidae family members. Methods Genomic DNA isolated from whole blood samples collected from 10 domestic cats and 45 big cats representing 11 species was subjected to PCR using primers flanking the coding regions for the activation peptides of PAR1, PAR3, and PAR4. PCR products were isolated from agarose gels and submitted for sequencing. Nucleotide sequence data was used to predict the amino acid composition of the activation peptide and flanking regions of the 3 receptors. Predicted amino acid sequences were compared between Felidae members and to human beings. Results Variations in the predicted amino acid composition of the activation peptides and flanking regions of the various PAR were observed when comparing Felidae family members to each other and to human beings. Conclusions While the activation peptide regions of the various PAR tend to be conserved, there are differences that may impact the ability of some agonists to mediate biased signaling events documented to occur in human platelets. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text