Fidelity of translation in the presence of mammalian mitochondrial initiation factor 3
| Autor: | Shreya Ahana Ayyub, Umesh Varshney, Srinivas Aluri, Linda L. Spremulli, S L Aswathy, Divya Dobriyal |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Genetics eIF2 Prokaryotic initiation factor-2 Prokaryotic initiation factor-3 Codon Initiator Prokaryotic Initiation Factor-3 Cell Biology EIF4A1 Biology Recombinant Proteins Eukaryotic translation initiation factor 4 gamma Mitochondrial Proteins 03 medical and health sciences 030104 developmental biology RNA Transfer Eukaryotic initiation factor Escherichia coli Humans Molecular Medicine Initiation factor Peptide Chain Initiation Translational Molecular Biology Prokaryotic initiation factor |
| Zdroj: | Mitochondrion. 39:1-8 |
| ISSN: | 1567-7249 |
| DOI: | 10.1016/j.mito.2017.08.006 |
| Popis: | Initiation factor 3 (IF3) is a conserved translation factor. Mutations in mitochondrial IF3 (IF3mt) have been implicated in disease pathology. Escherichia coli infCΔ55, compromised for IF3 activity, has provided an excellent heterologous system for IF3mt structure-function analysis. IF3mt allowed promiscuous initiation from AUA, AUU and ACG codons but avoided initiation with initiator tRNAs lacking the conserved 3GC pairs in their anticodon stems. Expression of IF3mt N-terminal domain, or IF3mt devoid of its typical N-, and C-terminal extensions improved fidelity of initiation in E. coli. The observations suggest that the IF3mt terminal extensions relax the fidelity of translational initiation in mitochondria. |
| Databáze: | OpenAIRE |
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