CTRP9 alleviates foam cells apoptosis by enhancing cholesterol efflux
| Autor: | Yuan Guo, Tingting Li, Shengyun Lei, Dan Xu, Anju Zuo, Jun Li, Ji-Ying Chen, Chengxiang Song |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
THP-1 Cells Apoptosis 030209 endocrinology & metabolism Biochemistry 03 medical and health sciences 0302 clinical medicine Endocrinology Downregulation and upregulation CYP27A1 Animals Humans cardiovascular diseases Wnt Signaling Pathway Molecular Biology Glycoproteins Liver X Receptors Foam cell biology Chemistry Hydrolysis Adenylate Kinase AMPK Biological Transport Lipid Metabolism Lipids Cell biology Mice Inbred C57BL PPAR gamma Cholesterol 030104 developmental biology ABCG1 ABCA1 biology.protein lipids (amino acids peptides and proteins) Adiponectin Efflux Receptors Adiponectin ATP Binding Cassette Transporter 1 Foam Cells |
| Zdroj: | Molecular and Cellular Endocrinology. 522:111138 |
| ISSN: | 0303-7207 |
| Popis: | The apoptosis of foam cells leads to instability of atherosclerotic plaques. This study was designed to explore the protective role of CTRP9 in foam cell apoptosis. In our experiment, CTRP9 alleviated foam cell apoptosis. Meanwhile, CTRP9 upregulated the expression of proteins important for cholesterol efflux, such as LXRα, CYP27A1, ABCG1 and ABCA1, and improved cholesterol efflux in foam cells. Moreover, CTRP9 inhibited Wnt3a and β-catenin expression and β-catenin nuclear translocation in foam cells. In addition, adenovirus overexpression of Wnt3a abolished the effect of CTRP9 on macrophage apoptosis. Mechanistically, the AMPK inhibitor abolished the effect of CTRP9 on foam cell apoptosis, and downregulation of AdipoR1 by siRNA abrogated the activation of AMPK and the effect of CTRP9 on foam cell apoptosis. We concluded that CTRP9 achieved these protective effects on foam cells through the AdipoR1/AMPK pathway. |
| Databáze: | OpenAIRE |
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