Effect of total parenteral nutrition, systemic sepsis, and glutamine on gut mucosa in rats
| Autor: | S. Yoshida, Kevin T. Bush, T. P. Stein, S. Lanza-Jacoby, Robert G. Nagele, M. J. Leskiw, M. D. Schluter |
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| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_specialty
Physiology Glutamine Endocrinology Diabetes and Metabolism Muscle Proteins Ileum Biology Infections Jejunum Sepsis Intestinal mucosa Physiology (medical) Internal medicine medicine Animals Intestinal Mucosa Rats Inbred Strains Metabolism medicine.disease Rats Microscopy Electron medicine.anatomical_structure Endocrinology Parenteral nutrition Female Parenteral Nutrition Total Leucine |
| Zdroj: | American Journal of Physiology-Endocrinology and Metabolism. 263:E368-E373 |
| ISSN: | 1522-1555 0193-1849 |
| DOI: | 10.1152/ajpendo.1992.263.2.e368 |
| Popis: | The effect of the combination of total parenteral nutrition (TPN) and systemic sepsis on mucosal morphology and protein synthesis was investigated. Rats were given a standard TPN mixture consisting of glucose (216 kcal.kg-1.day-1), lipid (24 kcal.kg-1.day-1), and amino acids (1.5 g N.kg-1.day-1) for 5 days. On the 5th day the rats (n = 37) were randomized into four groups according to diet as follows: 1) control nonseptic on standard TPN, 2) control nonseptic on TPN with glutamine, 3) septic on standard TPN, and 4) septic with the TPN supplemented with glutamine. Twenty hours after the injection of Escherichia coli, the rats were given a 4-h constant infusion of [U-14C]leucine to determine the mucosal fractional protein synthesis rates. The following results were obtained. 1) Histological examination showed that systemic sepsis caused tissue damage to the ileum and jejunum. 2) Glutamine supplementation attenuated these changes. 3) There were no visible changes to the colon either from glutamine supplementation or sepsis. 4) Sepsis was associated with an increase in mucosal protein synthesis and decreased muscle synthesis. 5) Addition of glutamine to the TPN mix further increased protein synthesis in the intestinal mucosa of septic rats. |
| Databáze: | OpenAIRE |
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