A cyclic AMP response element is involved in retinoic acid-dependent RARβ2 promoter activation
| Autor: | Frank A.E. Kruyt, Gert E. Folkers, Pt Vandersaag, Fae Kruyt, Ce Vandenbrink |
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| Rok vydání: | 1992 |
| Předmět: |
Receptors
Retinoic Acid TATA box Molecular Sequence Data Response element Retinoic acid Gene Expression Tretinoin Regulatory Sequences Nucleic Acid Kidney CREB Cell Line chemistry.chemical_compound Cyclic AMP Genetics Cyclic AMP Response Element-Binding Protein Humans Promoter Regions Genetic Transcription factor Base Sequence biology DNA TATA Box Molecular biology Retinoic acid receptor chemistry embryonic structures Mutagenesis Site-Directed biology.protein Cyclic AMP Response Element Carrier Proteins |
| Zdroj: | Nucleic Acids Research. 20:6393-6399 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/20.23.6393 |
| Popis: | Activation of the retinoic acid receptor (RAR) beta 2 promoter is known to be mediated by a RA response element located in the proximity of the TATA-box. By deletion studies in P19 embryonal carcinoma cells we have analyzed the RAR beta 2 promoter for the presence of additional regulatory elements. We found that the cyclic AMP response element-related motif, TGATGTCA at position -99 to -92, is able to enhance RA-dependent RAR beta 2 promoter activation. In addition we demonstrate that this element, designated CRE-beta 2, is functionally active as a CRE since it can bind members of the CREB/ATF transcription factor family and, moreover, mediates the stimulatory effect of cAMP on RA-dependent RAR beta 2 promoter activation in human foetal kidney 293 cells. |
| Databáze: | OpenAIRE |
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