DMP1 C-terminal mutant mice recapture the human ARHR tooth phenotype

Autor: Stephanie Lauziere, Anne Poliard, Yongbo Lu, Yixia Xie, Zhengguo Cao, Chunlin Qin, Baichun Jiang, Jian Q. Feng, Carol Janik, Leanne M Ward
Rok vydání: 2010
Předmět:
Genetically modified mouse
DMP1
medicine.medical_specialty
Dentinogenesis imperfecta
Endocrinology
Diabetes and Metabolism
Transgene
Mutant
Biology
medicine.disease_cause
Mice
odontoblast
03 medical and health sciences
0302 clinical medicine
stomatognathic system
Internal medicine
medicine
Animals
Humans
Orthopedics and Sports Medicine
Transgenes
In Situ Hybridization
DNA Primers
030304 developmental biology
hypophosphatemia
Extracellular Matrix Proteins
FGF-23
0303 health sciences
Mutation
Base Sequence
Reverse Transcriptase Polymerase Chain Reaction
030206 dentistry
medicine.disease
Molecular biology
Phenotype
Mice
Mutant Strains
Disease Models
Animal
Fibroblast Growth Factor-23
Endocrinology
Odontoblast
ARHR
Original Article
Familial Hypophosphatemic Rickets
Tomography
X-Ray Computed
Tooth
Zdroj: Journal of Bone and Mineral Research
ISSN: 0884-0431
DOI: 10.1002/jbmr.117
Popis: DMP1 mutations in autosomal recessive hypophosphatemic rickets (ARHR) patients and mice lacking Dmp1 display an overlapping pathophysiology, such as hypophosphatemia. However, subtle differences exist between the mouse model and human ARHR patients. These differences could be due to a species specificity of human versus mouse, or it may be that the mutant DMP1 in humans maintains partial function of DMP1. In this study we report a deformed tooth phenotype in a human DMP1 deletion mutation case. Unexpectedly, the deletion of nucleotides 1484 to 1490 (c.1484_1490delCTATCAC, delMut, resulting in replacement of the last 18 residues with 33 random amino acids) showed a severe dentin and enamel defect similar to a dentinogenesis imperfecta (DI) III–like phenotype. To address the molecular mechanism behind this phenotype, we generated delMut transgenic mice with the endogenous Dmp1 gene removed. These mutant mice did not recapture the abnormal phenotype observed in the human patient but displayed a mild rachitic tooth phenotype in comparison with that in the Dmp1-null mice, suggesting that the DI III–like phenotype may be due to an as-yet-undetermined acquired gene modifier. The mechanism studies showed that the mutant fragment maintains partial function of DMP1 such as stimulating MAP kinase signaling in vitro. Last, the in vitro and in vivo data support a role of odontoblasts in the control of fibroblast growth factor 23 (FGF-23) regulation during early postnatal development, although this regulation on Pi homeostasis is likely limited. © 2010 American Society for Bone and Mineral Research.
Databáze: OpenAIRE
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