Primary neurons can enter M-phase
| Autor: | Estíbaliz Barrio-Alonso, Wei Zhang, José María Frade, Iris Patiño-Parrado, Juan José Garrido, Chaska Carlos Walton |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Ministerio de Educación, Cultura y Deporte (España) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
G2 Phase Cyclin E Cell cycle checkpoint Cell division lcsh:Medicine Apoptosis Cell Cycle Proteins Article 03 medical and health sciences 0302 clinical medicine Humans lcsh:Science Mitosis Cytokinesis Neurons Oncogene Proteins Multidisciplinary biology Cyclin-dependent kinase 2 Cyclin-Dependent Kinase 2 lcsh:R Cell Differentiation Cell cycle Protein-Tyrosine Kinases Axon initial segment Cell biology 030104 developmental biology nervous system biology.protein lcsh:Q Tumor Suppressor Protein p53 030217 neurology & neurosurgery Cell Division |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-15 (2019) Digital.CSIC. Repositorio Institucional del CSIC instname Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-40462-4 |
| Popis: | Differentiated neurons can undergo cell cycle re-entry during pathological conditions, but it remains largely accepted that M-phase is prohibited in these cells. Here we show that primary neurons at post-synaptogenesis stages of development can enter M-phase. We induced cell cycle re-entry by overexpressing a truncated Cyclin E isoform fused to Cdk2. Cyclin E/Cdk2 expression elicits canonical cell cycle checkpoints, which arrest cell cycle progression and trigger apoptosis. As in mitotic cells, checkpoint abrogation enables cell cycle progression through S and G2-phases into M-phase. Although most neurons enter M-phase, only a small subset undergo cell division. Alternatively, neurons can exit M-phase without cell division and recover the axon initial segment, a structural determinant of neuronal viability. We conclude that neurons and mitotic cells share S, G2 and M-phase regulation. This study was supported by Ministerio de Economía y Competitividad grant SAF2015-68488-R (J.M.F.), SAF2015-65315-R (J.J.G.), and Ministerio de Educación, Cultura y Deporte grant FPU1305084 (C.C.W.). |
| Databáze: | OpenAIRE |
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