The consequences of multiple simulatancous C-type lectin-ligand interactions: DCIR alters the endo-lysosomal routing of DC-SIGN
| Autor: | García-Vallejo, Juan J, Bloem, Karien, Knippels, Léon M J, Garssen, Johan, van Vliet, Sandra J, van Kooyk, Yvette, Sub General Pharmacology, Sub Immunopharmacology, Pharmacology |
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| Přispěvatelé: | Molecular cell biology and Immunology, CCA - Immuno-pathogenesis, Sub General Pharmacology, Sub Immunopharmacology, Pharmacology |
| Rok vydání: | 2015 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
Glycan media_common.quotation_subject education Immunology antigen uptake macrophage galactose-type lectin C-type lectin Immunology and Allergy Macrophage dendritic cell immunoreceptor (DCIR) Dendritic Cells (DC) dendritic cells Internalization Receptor media_common Original Research mannose receptor biology dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) Cell biology macrophage galactose-type lectin (MGL) DC-SIGN dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin dendritic cell immunoreceptor biology.protein Mannose receptor (MR) intracellular routing lcsh:RC581-607 Intracellular Mannose receptor |
| Zdroj: | Frontiers in Immunology Frontiers in Immunology [E], 6. Frontiers Media S.A. Frontiers in Immunology, Vol 6 (2015) Frontiers in Immunology: Molecular Innate Immunity, 6:87. Frontiers Media S.A. Garcia Vallejo, J J, Bloem, K, Knippels, L M J, Garssen, J, van Vliet, S J & van Kooyk, Y 2015, ' The consequences of multiple simulatancous C-type lectin-ligand interactions: DCIR alters the endo-lysosomal routing of DC-SIGN ', Frontiers in Immunology: Molecular Innate Immunity, vol. 6, 87 . https://doi.org/10.3389/fimmu.2015.00087 |
| ISSN: | 1664-3224 |
| DOI: | 10.3389/fimmu.2015.00087 |
| Popis: | Antigen-presenting cells (APCs) are equipped with multiple receptors to allow proper pathogen recognition and capture. C-type lectin receptors (CLRs) recognize glycan struc- tures on pathogens and endogenous glycoproteins for internalization and antigen process- ing and presentation. Often, the glycan specificity of these receptors is overlapping and/or pathogens are decorated with ligands for multiple CLRs, posing the question whether interference or cooperativity within the CLR family exists. Here, we used imaging flow cytometry to investigate the internalization properties of four different CLRs (mannose receptor, DC-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN), macrophage galactose-type lectin, and dendritic cell immunoreceptor (DCIR)) on different APCs, as well as their intracellular routing. Although the internalization score of the inves- tigated CLRs was similar on monocytes, macrophages, and dendritic cells (DCs), DCIR internalization rates were lower compared to the other CLRs. Upon triggering, DCIR routed to intracellular compartments outside of the classical endo-lysosomal pathway, resulting in poor CD4 C T-cell stimulation. Although DC maturation reduced CLR expression levels, it did not affect their internalization rates. Although CLR internalization appeared to be independently regulated, DC-SIGN routing was affected when DCIR was triggered simul- taneously. In conclusion, our results provide new insights for the design of DC-based immunotherapeutic strategies and suggest that DCIR is an inferior target in this respect. |
| Databáze: | OpenAIRE |
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