Effect of alteration of translation error rate on enzyme microheterogeneity as assessed by variation in single molecule electrophoretic mobility and catalytic activity
| Autor: | Elnaz Shadabi, Douglas B. Craig, Ellert R. Nichols |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Mutant
medicine.disease_cause Biochemistry Streptozocin Capillary electrophoresis medicine Protein biosynthesis Escherichia coli Enzyme inducer Molecular Biology chemistry.chemical_classification biology Chemistry Ribosomal Protein S9 Escherichia coli Proteins beta-Glucosidase Translation (biology) Cell Biology Electrophoresis Enzyme Enzyme Induction Protein Biosynthesis biology.protein Biocatalysis Electrophoresis Polyacrylamide Gel |
| Zdroj: | Biochemistry and cell biology = Biochimie et biologie cellulaire. 87(3) |
| ISSN: | 0829-8211 |
| Popis: | The role of translation error for Escherichia coli individual β-galactosidase molecule catalytic and electrophoretic heterogeneity was investigated using CE-LIF. An E. coli rpsL mutant with a hyperaccurate translation phenotype produced enzyme molecules that exhibited significantly less catalytic heterogeneity but no reduction of electrophoretic heterogeneity. Enzyme expressed with streptomycin-induced translation error had increased thermolability, lower activity, and no significant change to catalytic or electrophoretic heterogeneity. Modeling of the electrophoretic behaviour of β-galactosidase suggested that variation of the hydrodynamic radius may be the most significant contributor to electrophoretic heterogeneity. |
| Databáze: | OpenAIRE |
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