Assays for the quantification of melphalan and its hydrolysis products in human plasma by liquid chromatography-tandem mass spectrometry
| Autor: | Jérôme Guitton, Bruno Vignal, Asmae Mirkou, Marc Guillaumont, Olivier Glehen, Sabine Cohen |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Electrospray ionization Clinical Biochemistry Mass spectrometry Biochemistry High-performance liquid chromatography Sensitivity and Specificity Analytical Chemistry Matrix (chemical analysis) Drug Stability Liquid chromatography–mass spectrometry Tandem Mass Spectrometry Humans Solid phase extraction Least-Squares Analysis Melphalan Chromatography High Pressure Liquid Aged Chromatography Chemistry Hydrophilic interaction chromatography Hydrolysis Selected reaction monitoring Solid Phase Extraction Reproducibility of Results Cell Biology General Medicine |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 877(27) |
| ISSN: | 1873-376X |
| Popis: | Two high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) assays are described for the quantification of melphalan in human plasma. N-phenyldiethanolamine was tested as internal standard. The first assay consisted of a protein precipitation by cold methanol and a reversed-phase HPLC whereas the second one was based on a solid phase extraction and a hydrophilic interaction chromatography. Both provided a very satisfactory mean extraction yield with a small volume of sample. The first method was simple, rapid and used as a routine assay. The second one was developed in order to determine melphalan hydrolysis products and to avoid scarce cases when interferences from biological matrix alter the quantification of melphalan using the first method. The two assays were linear and sensitive in the range of 1-500ng/mL for the first one and in a range of 25-2000ng/mL for the second one. Concentrations out of the range fixed with the first method were also validated. The procedure was reliable with precision and accuracy below 10%. All compounds were detected after positive mode electrospray ionization in selected reaction monitoring mode. These new analytical procedures were developed for melphalan pharmacokinetic studies or therapeutic drug monitoring. |
| Databáze: | OpenAIRE |
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