Lamellar and hexagonal lipid phases visualized by freeze-etching
| Autor: | Robert L. Leonard, David W. Deamer, Daniel Branton, Annette Tardieu |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
Chloroplasts
Materials science Fracture (mineralogy) Biophysics Oleic Acids Palmitic Acids Biochemistry law.invention X-Ray Diffraction law Particulate material Animals Lamellar structure Phospholipids Ovum Freeze Etching Hexagonal crystal system Degree of saturation Fatty Acids Lysophosphatidylcholines Membranes Artificial Cell Biology Plants Microscopy Electron Crystallography Cholesterol Membrane Strontium Phosphatidylcholines Calcium Female Electron microscope Chickens Stearic Acids |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Biomembranes. 219:47-60 |
| ISSN: | 0005-2736 |
| DOI: | 10.1016/0005-2736(70)90060-x |
| Popis: | 1. 1. Rapid freezing of lipid-water preparations preserves the structure of the high temperature phases. Both lamellar and hexagonal phases can be readily demonstrated by electron microscopy and X-ray observations. 2. 2. Without careful controls, contaminants are readily deposited on fresh fracture faces. The contamination can take the form of particles resembling those found on natural membranes. 3. 3. Neither degree of saturation, degree of hydration, nor cholesterol admixture significantly affects the appearance of lamellar fracture faces which appear uniformly smooth. On uncontaminated specimens, no structures were found which resembled the particulate material of natural membranes. |
| Databáze: | OpenAIRE |
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