Laminar Shear Stress–Induced GRO mRNA and Protein Expression in Endothelial Cells
| Autor: | Hideyuki Hagiwara, Xin Jin, Masako Mitsumata, Yoji Yoshida, Tetsu Yamane |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Male
Endothelium Chemokine CXCL1 Chemokine CXCL2 Biology Umbilical vein Physiology (medical) Shear stress medicine Animals Humans RNA Messenger Growth Substances Peptide sequence Aorta Cells Cultured Messenger RNA Chemotactic Factors cDNA library Middle Aged Molecular biology Neoplasm Proteins Endothelial stem cell medicine.anatomical_structure Gene Expression Regulation Cell culture Immunology Intercellular Signaling Peptides and Proteins Cattle lipids (amino acids peptides and proteins) Endothelium Vascular Stress Mechanical Chemokines Cardiology and Cardiovascular Medicine Chemokines CXC |
| Zdroj: | Circulation. 98:2584-2590 |
| ISSN: | 1524-4539 0009-7322 |
| DOI: | 10.1161/01.cir.98.23.2584 |
| Popis: | Background —The shear stress induced by blood flow may play a pivotal role in the induction or prevention of atherosclerosis by changing endothelial functions. To disclose the mechanisms of this change, we prepared an endothelial cell (EC) cDNA library to select specific clones expressed in response to shear stress. Methods and Results —The mRNA of cultured confluent bovine aortic ECs (BAECs) subjected to steady laminar shear stress (30 dyne/cm 2 ) for 4 hours was separated, and a cDNA library was prepared. Nine clones whose expressions were specifically enhanced by the shear stress were selected by use of a differential hybridization method. One clone had 94% homology at the nucleotide sequence level to Oryctolagus cuniculus gro (GRO) mRNA and 79% homology at the amino acid sequence level to human GRO-β. The GRO mRNA expression was increased in both BAECs and human umbilical vein ECs (HUVECs) after the ECs were subjected to high (30 dyne/cm 2 ) and low (5 dyne/cm 2 ) laminar shear stress. GRO-α and/or -β protein expression also increased after the HUVECs and BAECs were subjected to shear stress. Because GRO protein has been shown to function as an adhesion factor of monocytes on the surface of ECs, we studied whether shear stress–induced monocyte adhesion was caused by GRO protein expression on ECs. The 4-hour shear stress enhanced monocyte adhesion to ECs by 2.5-fold over control levels, and this enhancement was inhibited by 53% by anti–GRO-α antibody. Conclusions —The present study is the first report that shear stress induced the expression of GRO mRNA and protein in ECs and enhanced the monocyte adhesion on ECs via GRO protein. Further investigations of the functions and participation in atherogenesis of this selected clone may clarify the significance of shear stress on atherogenesis. |
| Databáze: | OpenAIRE |
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