Utility of oligoclonal IgG band detection for MS diagnosis in daily clinical practice
| Autor: | J. M. Ferrer, Esther Rodríguez, J.J. Rodríguez-Molina, V. Nuñez, E. Ramil, Guillermo Izquierdo, Margarita López-Trascasa, J.A. García-Trujillo, J.C. Álvarez-Cermeño, Maria I de José Gómez, Carmen Espejo, Xavier Montalban, David G. Munoz, Celia Oreja-Guevara, A. Gosis, A. Muriel, A. García-Merino, Alvaro Prada, Bonaventura Casanova, Oscar Fernandez, Eduardo Ruiz, R. Arroyo, Alfredo Rodríguez-Antigüedad, Luisa M. Villar, Jorge Jimenez, Carmen Cámara, Albert Saiz, Maria Isabel García-Sánchez, V. Abraira, C. de Andrés, Maria Jose Moreno, José Antonio Navarro, Claudio E Rodriguez, C. González-Oria, E. Sarasola, J. Yagüe, S. Cubillo, Magdolna Simó, Javier Olascoaga, M. A. Figueredo, Francisco Rodriguez-Frias, C. Ramo-Tello |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
Pathology Multiple Sclerosis Concordance Blotting Western Immunology Oligoclonal IgG Sensitivity and Specificity Gastroenterology Immunoenzyme Techniques Internal medicine Humans Immunology and Allergy Medicine False Positive Reactions False Negative Reactions Immunoassay Observer Variation Reproducibility business.industry Multiple sclerosis Oligoclonal Bands medicine.disease Clinical Practice Multicenter study Spain Immunoglobulin G business Kappa |
| Zdroj: | Journal of Immunological Methods. 371:170-173 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/j.jim.2011.06.009 |
| Popis: | An early and accurate diagnosis of multiple sclerosis (MS) is very important, since it allows early treatment initiation, which reduces the activity of the disease. Oligoclonal IgG band (OCGB) detection is a good ancillary tool for MS diagnosis. However, it was argued that its usefulness was limited by the high interlaboratory variability. In the last years, different techniques for OCGB detection have appeared. We performed a blinded aleatorized multicenter study in 19 Spanish hospitals to assess the accuracy and reproducibility of OCGB detection in this new scenario. We studied cerebrospinal fluid (CSF) and serum samples from 114 neurological patients. Every hospital contributed to the study with triplicated pairs of CSF and serum samples of six patients and analyzed 18 different samples. Global analysis rendered a sensitivity of 92.1%, a specificity of 95.1% and a Kappa value of 0.81. This shows that current techniques for OCGB detection have good accuracy and a high interlaboratory reproducibility and thus, represent a good tool for MS diagnosis. When we analyzed separately the different techniques used for OCGB detection, the highest concordance was observed in western blot with alkaline phosphatase detection (kappa=0.91). This indicates that high sensitivity techniques improve the reproducibility of this assay. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect