Exploration of α-pinene degradation pathway of Pseudomonas rhodesiae CIP 107491. Application to novalic acid production in a bioreactor
| Autor: | Pierre Fontanille, Denis Linares, Christian Larroche |
|---|---|
| Přispěvatelé: | Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
0106 biological sciences
0303 health sciences [SDV.BIO]Life Sciences [q-bio]/Biotechnology biology Bioconversion Chemistry [SDE.IE]Environmental Sciences/Environmental Engineering Pseudomonas biology.organism_classification 7. Clean energy 01 natural sciences [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology 03 medical and health sciences Metabolic pathway 010608 biotechnology Yield (chemistry) Pseudomonas rhodesiae Bioreactor Organic chemistry [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering Isomerization Cis–trans isomerism ComputingMilieux_MISCELLANEOUS 030304 developmental biology Food Science |
| Zdroj: | Food Research International Food Research International, 2009, 42 (4), pp.461-469. ⟨10.1016/j.foodres.2008.12.001⟩ Food Research International, Elsevier, 2009, 42 (4), pp.461-469. ⟨10.1016/j.foodres.2008.12.001⟩ |
| ISSN: | 0963-9969 |
| DOI: | 10.1016/j.foodres.2008.12.001⟩ |
| Popis: | α-Pinene degradation pathway of Pseudomonas rhodesiae CIP 107491, which includes flavoring molecules like cis-2-methyl-5-isopropylhexa-2,5-dienal (isonovalal) and trans-2-methyl-5-isopropylhexa-2,5-dienoic acid (novalic acid) has been extensively studied. Studies took into account biotic and abiotic degradation phenomena, which involved isonovalal and its trans isomer, novalal. Data showed that gas stripping of aldehydes, a spontaneous autooxidation of isonovalal into isonovalic acid (cis form of novalic acid) and a cofactor-dependent isomerization of isonovalal into novalal occurred. Novalic acid was not an end product and was transformed into 3,4-dimethylpentanoic acid by fresh (non permeabilized) cells with a production yield of 40%, while isonovalic acid was not consumed. A three main steps metabolic scheme was outlined to explain α-pinene oxide bioconversion into novalic acid. These data enabled to perform an optimized bioconversion of α-pinene oxide into novalic acid in a stirred aerated tank bioreactor in biphasic conditions. Results allowed to obtain c.a. 16 g/L product in about 8 h with a yield close to 40%. |
| Databáze: | OpenAIRE |
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